卷叶黄精根中甾体皂苷化学成分及其抗菌活性

Steroid Saponins of Polygonatum cirrhifolium Root and Their Antisepsis Activity

利用硅胶、RP-18和Sephadex LH-20等色谱分离手段从卷叶黄精根茎的正丁醇萃取物(总甾体皂苷R)中分离得到2个甾体皂苷化合物,通过理化性质、波谱分析及与文献值对照,鉴定化合物Ⅰ为(25R)-spirost-5-ene-3β-ol-3-O-α-L-rham-nopyranosy(1→2)-[α-L-rhamnopyranosyl(1→4)]-β-D-glucopyranoside,化合物Ⅱ为(25R)-spirost-5-ene-3β-ol-3-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranoside。将化合物Ⅰ进行双相酸水解得到其苷元(化合物Ⅲ),鉴定为薯蓣皂苷元。对总甾体皂苷R、化合物Ⅰ、Ⅱ及Ⅲ的抗菌活性进行测定,结果表明:总甾体皂苷R、化合物Ⅰ及Ⅱ对植物病原菌的抑制效果均比对细菌的抑制效果明显,而化合物Ⅲ对细菌的抑制效果均比对植物病原菌强。

Steroid saponins（R）was extracted from the Polygonatum cirrhifolium root.Two compounds were isolated and purified by the combined use of column chromatography with silical gel,RP-18 and SephadexLH-20 methods.The structure of the compound were elucidated by spectroscopic and the data reported in literature methods.Two compounds were identified as（25R）-spirost-5-ene-3β-ol-3-O-α-L-rham-nopyranosy（1→2）-[α-L-rhamnopyranosyl（1→4）]-β-D-glucopyranoside（Ⅰ）and（25R）-spirost-5-ene-3β-ol-3-O-α-L-rhamnopyranosyl（1→4）-β-D-glucopyranoside（Ⅱ）.The aglycones of compound Ⅰ was obtained by the method of two-phase acid hydrolysis and its structure was identified as diosgenin（Ⅲ）.The antisepsis activity of R,compound Ⅰ,Ⅱ and Ⅲ were studied,the results were as follows：Steroid saponins R,compound Ⅰ and Ⅱ had strong inhibition against plant pathogenic fungi growth than bacteria＇s,but compound Ⅲ had strong inhibition against to bacteria growth than plant pathogenic fungi＇s.