固黄O-CTMAB-DNA三元体系的共振光散射光谱的研究及应用

Resonance Light Scattering Spectrophtometic Determination of Trace DNA with the System of FYO-CTMAB and Its Analytical Application

研究了脱氧核糖核酸(DNA)与阴离子染料固黄O(FYO)及阳离子表面活性剂溴化十六烷基三甲铵(CTMAB)三元体系的共振光散射光谱的特征.考察了各种影响因素,在优化条件下确立了共振光散射强度与鱼精DNA和小牛胸腺DNA以及变性后的鱼精DNA和小牛胸腺DNA浓度之间的关系,相应的线性范围分别为0.026～7.00mg/L;0.025～7.00mg/L;0.032～6.00mg/L;0.031～8.00mg/L,相关系数为0.9991;0.9996;0.9981;0.9996,检出限分为7.0,6.0,11.0,12.5μg/L,基于共振光散射的增强,建立了一种测定DNA的新方法.

Resonance light-scattering （RLS） spectra characteristics of interaction between DNA and system of FYO- CTMAB was studied. The effects of interference substances and optimization of procedure were investigated and a new method was established for the determination of the fsDNA, ctDNA, denaturation fsDNA, and denaturation ctDNA at nanogram level based on RLS measurement. The linear range the determination are 0.025～7.00 mg/L; 0.025～7.00 mg/L; 0.032～5.00 mg/L; 0.031～8.00 mg/L and the correlation coefficient are 0.999 1 ; 0.999 5; 0.998 1 ; 0.999 5. The detection limit are 7.0, 5.0, 11.0, 12.5μg/L. The method was applied to determination of DNA in mixed samples with satisfactory result. 5figs., 4tabs., 8refs.