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嗜肺军团菌感染后血清白介素12水平和巨噬细胞功能的变化 被引量：3

The changes of serum IL-12 and the killing function of macrophages after legionella pneumophia infection
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摘要 目的观察嗜肺军团菌感染BALB/C小鼠后血清白介素12(IL-12)水平和巨噬细胞功能的变化,探讨嗜肺军团菌感染后对机体免疫功能的影响。方法BALB/C小鼠腹腔注射嗜肺军团菌菌液0.2ml,菌液浓度为1×106/ml,利用标准试剂盒酶联免疫吸附检验(ELISA)和巨噬细胞吞噬实验观察血清IL-12水平和巨噬细胞吞噬功能的变化。结果嗜肺军团菌感染前小鼠血清IL-12为(19.61±3.57)μg/ml,感染1周后为(15.35±2.87)μg/ml(P<0.01);感染前腹腔巨噬细胞吞噬功能为58.63±9.37,感染1周后为32.51±6.96(P<0.01);感染4周后,血清IL-12水平和巨噬细胞吞噬功能与感染前无显著性差异。结论嗜肺军团菌感染1周后机体免疫功能明显下降,4周后免疫功能基本恢复到正常水平。 Objective To study the changes of serum level of interleukin-12（IL-12） and the killing function of macrophages after legionella pneurnophia infection in BALB/C mice and explore effect of legionella pneumophila infection on immunity. Methods Serum IL-12 and macrophage function were examined with ELISA and macrophage killing tests, respectively. Results IL-12 level in serum was decreased significantly from （19.61±3.57） μg/ml before infection to （15.35±2.87） μg/ml one week after infection（P〈0. 01）. So did the killing function of macrophage from mouse abdominal cavity（58.63±9.37 vs. 32.51±6.96） （P〈0.01）. IL-12 level and macrophage function were not significantly different between those before and 4 weeks after infection. Conclusion Immunity is markedly decreased one week after legionella pneumophilia infection, which is recovered to normal level 4 weeks after infection.
作者 王剑 诸葛洪祥 黄利红
机构地区 苏州大学病原生物学教研室
出处 《江苏医药》 CAS CSCD 北大核心 2007年第9期927-928,共2页 Jiangsu Medical Journal
关键词 嗜肺军团菌 白介素12 巨噬细胞 Legionella pneumophia Interleukin- 12 Macrophage
分类号 R517 [医药卫生—内科学]
  • 相关文献

参考文献6

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二级参考文献18

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共引文献7

同被引文献19

  • 1社区获得性肺炎诊断和治疗指南[J].中华结核和呼吸杂志,2006,29(10):651-655. 被引量：3052
  • 2张景,刘元明,张薇,王贝晗,郝钦芳,刘爱兵.血清铁蛋白测定对几种恶性肿瘤的诊断价值[J].武警医学,2007,18(1):74-75. 被引量：15
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引证文献3

二级引证文献7

江苏医药
2007年 第9期

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