一种简便分离高质量家蚕基因组DNA的方法

A simple method of isolating high quality Genomic dna from the silkworm

本研究对传统的家蚕基因组提取方法进行了改进。改进方法无需研钵和液氮,将RNase与蛋白酶K同时加入剪碎的家蚕样品中消化处理,并利用旋转振荡培养箱旋转混匀和培育每步的样品。该方法减少了基因组DNA的损失,提高了工作效率,节约了成本。利用改进后的方法对不同家蚕品种的基因组DNA进行了提取和PCR扩增验证,结果表明提取的基因组DNA的质量、获得率、重复性都更好,能够满足家蚕分子生物学研究的需要。

This study improved the conventional method for extracting genomic DNA from silkworm. The improved method does not need mortars and liquid nitrogen. Silkworm tissues pieces by scissors is digested in lysis buffer with RNase and proteinaseK, and mix and incubate every step by revolving and shaking incubator. It decrease the loss of genomic DNAs, and improve work efficiency, reduce cost. Genomic DNA was isolated from silkworms of different strains by this improved method, and was amplified by PCR. Results indicated that this improved method is more recovered rate, repeatability, quality than conventional method. This will suffice to research of silkworm in molecular biology.