养血清脑颗粒对小鼠的镇静催眠作用

Sedation effects of Yangxueqingnao Granual on mice

目的:观察养血清脑颗粒对小鼠的镇静催眠作用。方法:①50只昆明种雄性小鼠随机分为5组,即生理盐水对照组,阳性对照组及养血清脑颗粒大、中、小剂量组,每组10只,灌胃给药1次后采用抖笼换能器法测定小鼠的自主活动。②另取50只小鼠同法分组,连续灌胃给药3d,2次/d,于末次给药后50min腹腔注射30mg/kg戊巴比妥钠,记录睡眠时间。③另取150只小鼠,分组与给药方法同②,并于末次给药后50min腹腔注射25mg/kg戊巴比妥钠,观察30min后的入睡鼠数。结果:与生理盐水对照组相比,大、中剂量的养血清脑颗粒可减少小鼠的自发活动,大、中、小剂量的养血清脑颗粒与戊巴比妥钠合用可延长小鼠的睡眠时间(P<0.01),大、中、小剂量的养血清脑颗粒与阈下剂量戊巴比妥钠合用,入睡鼠数增加(P<0.05)。结论:养血清脑颗粒有明显的镇静催眠作用,且与戊巴比妥钠有协同作用。

Aim： To observe the sedation effects of Yangxueqingnao Granual on mice. Methods： Ⅰ ：Fifty mice were allocated into 5 groups： normal saline group, positive control grooup, and Yangxueqingnao Granual high-, middle-, low- dose groups, 10 in each group. After being administered by gavage, the spontaneous movement of mice was detected by tremble cage transducer, Ⅱ ： Another 50 mice were allocated into 5 groups as above. The mice were administered by gavage for 3 days, twice per day. At 50 min after the last administration, the pentobarbital sodium at 30 mg/kg was given. The sleeping time of mice was recorded. Ⅲ ： A total of 150 mice were allocated into 5 groups （30 in each group） and treated as method Ⅱ . At 50 min after the last administration, the pentobarbital sodium at 25 mg/kg was given. The sleeping mice was counted. Results： Yangxueqingnao Granual could reduce the spontaneous movement of mice. The combinatior of Yangxueqingnao Granual and pentobarbital sodium could prolong sleeping time and increase the number of sleeping mice. Conclusion：Yangxueqingnao Granual is effective in sedation especially in combination with pentobarbital sodium.