DTT与顺铂诱导对食管癌Eca109细胞磷酸化P38表达的影响

Relationship between the expression of p-P38 and apoptosis of Eca109 cells induced by cisplatin and DTT

目的:探讨磷酸化P38在顺铂和DTT诱导的食管癌Eca109细胞凋亡中的作用。方法:分别用DTT与顺铂诱导处理食管癌Eca109细胞,未加药组作为对照。采用流式细胞仪技术检测细胞凋亡率;用免疫组化方法检测磷酸化P38的表达情况。结果:与对照组相比,DTT处理组及顺铂处理组细胞凋亡率明显升高。免疫组化结果显示:2处理组磷酸化P38的水平均明显高于对照组(P<0.05);DTT处理组磷酸化P38大部分位于细胞核内,而顺铂组磷酸化P38则主要弥散于细胞浆内。结论:磷酸化P38在DTT和顺铂诱导的食管癌Eca109细胞凋亡时细胞内分布不同,激活的P38可能通过不同途径参与细胞凋亡。

Aim： To study the relationship between the expression of phosphorylated P38 （p-P38） and apoptosis of Eca109 cells induced by cisplatin and DTT. Methods：Esophageal carcinoma Eca109 cells were treated with either cisplatin or DTT. The untreated cells served as the control. The cell apoptosis was detecled by flow cytometry. The P38 phosphorylation was detected by immunohistochemistry. Results： The apoptotic rates of Eca109 cells in cisplatin and DTT treatment groups were higher than that of control group（P 〈 0.05）. The phosphorylation level of P38 in the 2 treatment groups was much higher than that of control group （P 〈 0.05）. DTT treatment, but not cisplatin treatment, induced nuclear translocation of p-P38. Conclusion： The localization of p-P38 is different in Eca109 cell apoptosis induced by cisplatin and DTT. P38 activation may be associated with multiple apoptosis pathways.