酶免法检测HBsAg中实验操作个体差异及可疑区域的界定

Differences between operators in testing HBsAg by ELISA and delimitation of dubious areas

目的:了解实验室内各操作个体应用酶联免疫吸附试验(酶免法)检测HBsAg的检测结果间的差异,为界定酶免法检测HBsAg的可疑区域提供实验数据。方法:10名实验室工作人员与全自动酶免检测仪分别对同一样本检测20次获得11组检测数据,运用q检验、u检验、方差分析及变异系数等统计技术对各组检测数据进行分析,并计算每个操作个体的检测可疑区域。结果:各实验操作个体的检测结果相比,F=13.36,P<0.01,变异系数在5.5%～17.8%,可疑区域的最大范围为64.4%～135.6%。结论:该试验中各实验操作个体的检测结果间存在较大差异,检测可疑区域的范围不能统一界定。

Aim： To find out differences between operators in testing HBsAg by ELISA and provide experimental data for delimiting the dubious areas. Methods： The data through testing the same sample twenty times by ten lab operators manually and by using automatic ELISA apparatus were collected and analyzed using statistical methods. Results： The data from different lab operators had significant difference （F = 13.36,P 〈 0.01 ）. CV ranged from 5.5% to 17.8% . The widest range for dubious areas was from 64.4% to 135.6%. Conclusion： There is a significant difference between different lab operators in experimental data and the range for dubious areas cannot be delimited generally.