不育症患者精浆男性抑制物质活性和抗精子抗体检测

Determination of male inhibition material activity and antisperm body in patients with infertility

目的:探讨不育症患者精浆男性抑制物(MIM)活性与抗精子抗体(ASAb)水平的变化。方法:非不育而需做精液检查者120例(对照组),年龄26～29岁;同期婚后2a以上、同居性生活正常不育者131例(不育组),年龄26～29岁;同期配偶流产组83例,年龄25～28岁。患者禁欲3～7d,以手淫方式取精液,在室温下自行液化做常规检查和测定。采用抗补体法检测MIM,采用ELISA试剂盒法检测ASAb。结果:对照组中119例MIM≥360U/mL,2例D(ASAb)>0.21。不育组中MIM200～U/mL33例,300～360U/mL98例;31例D(ASAb)>0.21,22例D(ASAb)>0.61;15例D(ASAb)>1.3。配偶流产组中MIM200～U/mL32例,300～360U/mL51例;18例D(AS-Ab)>0.21,12例D(ASAb)>0.61,7例D(ASAb)>1.3。3组精浆MIM活性、ASAb阳性强度比较差异均有统计学意义(P<0.05)。不育组患者精浆MIM活性与ASAb阳性强度呈负相关(r=-0.654,P<0.05)。结论:MIM活性降低、ASAb阳性强度增高可能是导致男性不育的原因。

Aim： To determine the male inhibition material （MIM） activity and anti-sperm antibodies （ASAb） level in semen from patients with infertility. Methods ：A total of 120 normal controls aging 26 - 29 years that required semen examination（ control group）, 131 patients with infertility, aging 26 - 29 years, with more than 2 years marriage and normal sex life（infertility group） , and 83 patients whose spouses got abortion, aging 25 -28 years（spouse abortion group） were investigated. The MIM activity and ASAb were determined using anti-complement method and ELISA, respectively. Results： In the control group, there were 119 cases with MIM≥360 U/mL and 2 cases with D （ASAb） 〉 0. 21. In infertility group, there were 33 cases with MIM 200 - U / mL, 98 cases with MIM 300 - 360 U/mL, 31 cases with D （ASAb） 〉 0. 21, 22 cases with D （ASAb） 〉 0. 61, and 15 cases with D （ASAb） 〉 1.3. In spouse abortion group, there were 32 cases with MIM 200 - U/mL, 51 cases with MIM 300 - 360 U/mL, 18 cases with D （ASAb） 〉0.21 , 12 cases with D （ ASAb） 〉0.61, and 7 cases with D （ASAb） 〉 1.3. The MIM activity and D （ASAb） in the 3 groups had significant differences （P 〈 0. 05 ）. The MIM activity was negatively correlated with D （ASAb） in infertility group （ r = - 0. 654, P 〈 0. 05）. Conclusion ： The decrease of MIM activity and increase of ASAb may result in infertility.