平阳霉素治疗后血管瘤组织中肿瘤坏死因子检测

Determination of TNF-α in hemangioma tissue after pingyangmycin sclerotherapy

目的:观察平阳霉素治疗后血管瘤的临床表现和生化变化,探索肿瘤坏死因子-α(TNF-α)在平阳霉素硬化疗法中的作用。方法:24例口腔颌面部血管瘤患者分为2组,分别将平阳霉素和鱼肝油酸钠用利多卡因以等体积稀释,在血管瘤周围正常皮肤黏膜处进针,穿刺瘤体回抽见血后,注入药物,隔10d注射1次,观察血管瘤体积的变化情况;分别于第1次注射后的第5天,第3次注射后的第10天,分2批手术切除取标本(每批每组各6例);对切取的标本行免疫组织化学染色检测TNF-α的表达情况。结果:鱼肝油酸钠注射后患者主诉血管瘤局部疼痛、肿胀明显,第3次注射后瘤体平均缩小50%,切除的组织内TNF-α均为阴性表达。平阳霉素注射后患者瘤体缩小明显,组织反应小,无明显疼痛不适,第3次注射后瘤体平均缩小80%,切除的组织内TNF-α均为阳性表达。结论:平阳霉素疗法效果好,肿瘤坏死因子可能是其发挥作用的一个重要机制。

Aim： To explore the role of TNF-α in pingyangmycin sclerotherapy of hemangioma, Methods： Twenty-four cases of oral-maxillofacial hemangioma were separated into two groups. The hemangiomas were injected with pingyangmycin or sodium morrhuate. Specimens were prepared for immunohistochemical observation on the 5th day after the 1st injection and the 10th day after the 3rd injection. Results： Pingyangmycin could reduce the size of hemangioma remarkably with minor inflammatory reaction. TNF-α in pingyangmycin group was positive. TNF-α in sodium morrhuate group was negative. Conclusion ： TNF-α plays an important role in pingyangmycin sclerotherapy of hemangioma.