凤尾兰提取物的抑菌活性及其对蒜薹腐烂的抑制作用

Antifungal Activity of Yucca gloriosa L.Extract and its Inhibitory Effect on the Rot of Garlic Bolt

灰葡萄孢和葱腐葡萄孢引起的蒜薹灰霉病是导致蒜薹贮期腐烂的主要病因之一。采用滤纸片扩散法研究了凤尾兰提取液对蒜薹灰葡萄孢和葱腐葡萄孢的抑制作用,用二倍稀释法确定提取液的最小抑菌浓度;在常温条件下贮藏,探讨不同浓度的凤尾兰提取物对蒜薹灰葡萄孢和葱腐葡萄孢引起腐烂的抑制作用。结果表明,凤尾兰提取液对灰葡萄孢和葱腐葡萄孢均有较明显的抑制作用,对两种菌的最小抑菌浓度分别为0.062 5 g/mL和0.125 g/mL。凤尾兰提取物对灰葡萄孢和葱腐葡萄孢引起的蒜薹腐烂具有较好的抑制、治疗作用,2.5 g/L、5 g/L、10 g/L的凤尾兰提取物均能有效抑制蒜薹灰葡萄孢和葱腐葡萄孢的侵染,减少蒜薹腐烂,其中以10 g/L的处理效果最佳。

Grey mold caused by Botrytis cinerea Pers （isolated from garlic bolt） and Botrytis allii Munn was one of the main reasons to the rot of garlic bolts during the storage. In this paper, the antifungal activity of Yucca gloriosa L. extract was determined by filter-disk diffusion method, the minimal inhibition concentration （MIC） of Yucca gloriosa L. extract was assayed by agar dilution method, and the inhibitory effect of Yucca gloriosa L. extract on the rot caused by Botrytis cinerea Pers and Botrytis allii Munn was studied at ambient. The results showed that the antifungal effect of Yucca gloriosa L. extract on Botrytis cinerea Pers and Botrytis allii Mun was significant, the MIC of Yucca gloriosa L. extract to Botrytis cinerea Pers and Botrytis allii Munn was 0.062 5 g/ mL and 0.125 g/mL, respectively. The inhibitory effect of Yucca gloriosa L. extract on the rot caused by Botrytis cinerea Pers and Botrytis allii Munn was significant, the invasion of the two fungus was inhibited efficiently by 2.5 g/L, 5 g/L, 10 g/L Yucca gloriosa L. extract and rot of garlic bolt was reduced, the best effect was attained by 10g/L in this three treatments.