TNFα、IL-2在肝癌冷冻免疫中的作用实验分析

Cryoimmunity experiment analysis live carcinoma

目的研究小鼠肝癌冷冻治疗后,体内免疫状况的变化,为冷冻免疫的临床应用提供免疫学理论依据。方法将腹腔内接种肝癌细胞的Ba1bs小鼠随机分为冷冻治疗组,手术切除组,比较各组手术前后免疫功能的变化情况。用双抗体夹心ELISA法,测其周围静脉血中TNFα和IL-2浓度。结果小鼠接种肝癌细胞后第15天即全部可见肿瘤生长,平均直径0.75cm,两组小鼠分别于手术后第14天、第21天、第28天取血检测TNFα及IL-2浓度,可见冷冻组指标均呈递增趋势,且较对照组明显增高(p<0.01),手术组其免疫指标无明显增高,且冷冻组肿瘤明显有缩小或坏死。多次重复上述实验,结果一致。结论肝细胞癌经冷冻治疗术后其免疫功能增强,存在着显著的冷冻免疫效应。

Objective To study immunocompetent changes in mice after cryosurgical treatment for liver carcinoma and collect immunological evidences for clinical application of cryosurgery. Methods The BALBS mice wire randomized into cryosurgery group and resection group after injection of HCC to abdominal cavity. Compare immunocompetent changes in two groups preoperatively and postoperatively . ELISA was applied to measure TNF-α,IL-2. Results Abdominal masses （an average diameter of 0.75cm）were observed in all mice on 15 days after injection. The two groups were treated by cryosurgery and resection respectively. Blood specimens were harvested to test relative immune guideline on14, 21 and 28 days after management. Tumors were apparently decreased or disappeared on 28 days in cryosurgery group . Immune criteria measurement indicated that TNFsand IL-2 were increased （p〈0.01）, The results kept the same after repeated of the processes. Conclusions Immunoenhancement can be achieved in HCC after cryosurgery, present apparently cryoimmunity effects.