摘要
Active volatile components in drug pair(DP)Herba Ephedrae-Ramulus Cinnamomi(HE-RC),single drug HE and RC were analyzed by gas chromatography/mass spectrometry(GC/MS),chemometric resolution method(CRM)and overall volume integration.By means of CRM,the two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound.In total,97,62,and 78 volatile chemical components in volatile oil of HE,RC,and DP HE-RC,were respectively determined qualitatively and quantitatively,accounting for 90.08%,91.62%,and 89.76% total contents of volatile oil of HE,RC,and DP HE-RC respectively.It is further demonstrated that the numbers of volatile components of DP HE-RC are almost the sum of those of two single drugs,but some relative contents of them are changed.Some new components,such as 1,6-dimethylhepta-1,3,5-triene,tetracyclo[4.2.1.1(2,5).0(9,10)]deca-3,7-diene,globulol and(E,E)-6,10,14-trimethyl-5,9,13-pentadecatrien-2-one are found in DP HE-RC because of chemical reactions and physical changes during decoction.
Active volatile components in drug pair （DP） Herba Ephedrae-Ramulus Cinnamomi （HE-RC）, single drug HE and RC were analyzed by gas chromatography/mass spectrometry （GC/MS）, chemometric resolution method （CRM） and overall volume integration. By means of CRM, the two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound. In total, 97, 62, and 78 volatile chemical components in volatile oil of HE, RC, and DP HE-RC, were respectively determined qualitatively and quantitatively, accounting for 90.08%, 91.62%, and 89.76% total contents of volatile oil of HE, RC, and DP HE-RC respectively. It is further demonstrated that the numbers of volatile components of DP HE-RC are almost the sum of those of two single drugs, but some relative contents of them are changed. Some new components, such as 1,6-dimethylhepta-1,3,5-triene, tetracyclo[4.2.1.1（2,5）.0（9,10）]deca-3,7-diene, globulol and （E,E）-6,10,14-trimethyl-5,9,13-pentadecatrien-2-one are found in DP HE-RC because of chemical reactions and physical changes during decoction.
基金
Project (01962502) supported by the Natural Science Foundation of Hunan Province, China
关键词
药物配对
化学分析
挥发油
分辨能力
drug pair （DP）
Herba Ephedrae-Ramulus Cinnamomi
chemometric resolution method （CRM） volatile oil
gas chromatography/mass spectrometry （GC-MS）
