啤酒酵母硫化氢生成量测定方法的研究

Study on the Determination of H_2S Produced by Saccharomyces cerevisiae

建立了一种啤酒酵母硫化氢生成量的测定方法。以pH为6.0的乙酸锌溶液作吸收液,采用250mL三角瓶进行发酵时,最适的发酵系统为:装液量150mL,发酵温度24℃,麦汁初始pH5.0,接种量6%。此方法可用于定量比较不同酵母菌株的硫化氢生成量。

One method of determination of H2S produced by Saccharomy ces cerevisiae was studied. With this method, Zn（At）2 was used as trapping solution whose pH was 6.0.Fermentation trials were conductated in 250mL conical flasks filled with 150ml inoculated medium.Flasks were incubated at 24℃ after inoculated with 6% inoculum and the pH was 5.0. The method can be used to compare the quantity of H2S produced by different strains.