对苯二胺对小鼠骨髓细胞致突变的影响

The Effect of PPD on Bone Marrow Cell Mutation in Mice

[目的]探讨对苯二胺(PPD)对小鼠骨髓嗜多染红细胞(PCE)致突变的影响。[方法]选择健康的昆明种小鼠50只,随机分为5组,每组10只。PPD高剂量组(215.00mg/kg),次高剂量组(107.50mg/kg),中剂量组(53.75 mg/kg),低剂量组(26.88mg/kg),采用腹腔注射0.2ml法染毒,每天1次,连续2d;给对照组注射生理盐水0.2ml。染毒后,测定并计算小鼠PCE的微核率;用单细胞凝胶电泳技术(SCGE)测定骨髓细胞的彗星细胞数和彗尾长度。[结果] PPD可致小鼠PCE微核率明显增加,引起拖尾细胞数相应增加。且都存在剂量-反应关系。[结论]PPD对小鼠PCE具有较强的致突变性。

[ Objective ] To investigate the effect of p-phenylenediamine （ PPD ）on bone marrow polychromatic erythrocyte （ PCE ）mutation in mice. [ Methods ] 50 mice were divided into five groups at random. 0.2 ml PPD in different dosage（ 215.00 mg/kg, 107.50 mg/kg, 53.75 mg/kg and 26.88 mg/kg respectively ）were injected intraperit0neally daily for 2 days in 4 different test groups of mice. 0.2 ml normal saline was injected in the control group only. The number of micronucleus in PCE of bone marrow were measured after treated with this hair dye and the number of comet and its tail length were determined using single-cell gel eiectrophoresis（ SCGE ）. [ Results ] It is found that the PPD could increase the micronucleus rate obviously and a dose-effect relationship between PPD and micronucleus rate existed. [ Conclusion ] PPD had strong mutagenic effect on bone marrow PCE mutation in mice.