PUVA照射与青黛丸口服对银屑病患者血cAMP、cGMP、SOD及LPO含量的影响

The Effect of PUVA and Qing Dai Pill on SOD, LPO, cAMP and cGMP Levels in Psoriatic Patients

目的：研究ＰＵＶＡ及青黛丸口服治疗寻常型银屑病对患者血液中ＳＯＤ活力、ＬＰＯ水平、ｃＡＭＰ及ｃＧＭＰ含量的影响。方法：寻常型银屑病患者７４例，随机分成ＰＵＶＡ照射组（３６例）及青黛丸服用组（３８例），按常规行治疗方案。采用微量指血超氧化物歧化酶（ＳＯＤ）快速测定法测定红细胞内ＳＯＤ活力，荧光分光光度法测定血清脂质过氧化物（ＬＰＯ）值、放射免疫分析法测定血浆ｃＡＭＰ及ｃＧＭＰ含量。结果：治疗前患者ＳＯＤ活力皆低于对照组（Ｐ＜０．０１），ＬＰＯ水平无明显差异，ｃＡＭＰ低于对照组，而ｃＧＭＰ高于对照组。青黛丸口服后，ＳＯＤ活力改善，ｃＡＭＰ有所回升，但ＬＰＯ及ｃＧＭＰ变化不明显；ＰＵＶＡ照射后，ＳＯＤ活力进一步低于疗前水平，ＬＰＯ值则高于疗前，ｃＡＭＰ的改善略优于青黛丸，ｃＧＭＰ明显改善。对两种疗法的疗效、改变上述指标的可能作用机理及其与临床应用的相互关系作了分析。

The study compares the effect of PUVA and Qing Dai pill (a Chinese traditional medicine) regimen on SOD, LPO, cAMP and cGMP level variation in psoriatic patients. Seventy four patients with psoriasis vulgaris were classified into two treatment groups——PUVA (36 cases) and Qing Dai pill (38 cases). SOD activity in RBC was detected by rapid catechol assay with microvolume of finger blood; serum LPO level was determined by TBA fluorescence colorimetric assay; plasma cAMP and cGMP concentrations were measured by RIA technology. Before treatment SOD activity was lower in all patients in comparison with normal controls (P<0 01). LPO level was in normal range. cAMP was lower and cGMP higher than that of control group. After Qing Dai pill treatment, SOD activity and cAMP were improved to some degree, but LPO and cGMP levels did not show noticeable changes. After PUVA treatment, SOD activity further decreased and LPO increased. The therapeutic effect of PUVA on cAMP was a little better, but cGMP, much better than those of Qing Dai pill. The therapeutic effects of two treatments, possible mechanism of the effects on these biochemical indexes and their relation in clinical practice were discussed and analysed.