气相色谱法测定维生素 E 微乳中维生素 E 的含量

Quantitative Determination of Vitamin E in Vitamin E Microemulsion by GC

维生素Ｅ微乳由维生素Ｅ、吐温－８０、甘油及水组成，通过绘制相图筛选最佳配方及制备工艺；采用气相色谱法测定微乳中维生素Ｅ的含量，以３％ＯＶ－１７为固定相，８０～１００目１０１担体，柱温为２６５℃，以三十二烷为内标，线性范围为０．４～２．０ｍｇ，相关系数为０．９９５２，平均回收率为１０１．８％，ＲＳＤ＝０．１％。

A method for the quantitative determination of vitamin E in vitamine E microemulsion by gas chromatography was established with 3% OV 17 as liquid phase,n dotriacontane as internal standard and 101 (white,80￣100 mesh) as support,and with column temperature 265℃,injection port temprature 280℃ and FID detector. The standard curve showed linear relationship within the range of 0.4￣2.0 mg of microemulsion of vitamin E with correlation coefficient 0.9952,RSD 0.1% and mean recovery 101 8%. The method was simple and accurate.