实验性犬侧颅底骨缺损修复材料的研究

Histological study of experimental reconstructive materials for lateral skull base and dural defects in dogs

为达到修复颅底骨和硬脑膜缺损的目的，通过手术造成狗侧颅底骨及硬脑膜大面积缺损模型，利用自体阔筋膜或与细胞生长促进因子——骨形成蛋白（ｂｏｎｅｍｏｒｐｈｏｇｅｎｔｉｃｐｒｏｔｅｉｎ，ＢＭＰ）进行修复。观察移植后不同时期所修复组织的成活及成骨的组织学变化情况。探讨了阔筋膜修复硬脑膜后组织学变化，证实生物膜与骨形成蛋白是一种理想的颅底缺损修复材料，为临床修复硬脑膜、颅底缺损等提供了参考。

In order to observe the reconstructive effects of fascia lata,superficial fascia and bone morphogentic protein (BMP)in skull base surgery, a lateral skull base bone and dural defect model in dogs was established.Fascia was selected alone or combined with BMP as repairing materials and BMP was evaluated in reconstructing large cranial defect model .Forty dogs underwent a 3.0×4.0 cm full-thickness excision of the parietal bone were divided into different groups of five dogs each ,and were reconstructed by ①fascia lata; ②fascia lata plus BMP; ③nonreconstructed controls; ④fascia lata plus BMP and exposed .The implants were harvested at 2～15 weeks and examined histologically .The new bone was found in the BMP. Implants treated and untreated were quite different. The unreconstructed controls demonstrated only a bridge of fibrovasular connective tissue. BMP are defined as osteoinductive by their ability to produce bone formation by stimulating mesenchymal cells to tranform into bone producing osteo blasts.The results suggested the combination of BMP and reconstructive material in the treatment of bone defects.