摘要
目的研究儿童慢性HBV携带者的T细胞亚群状态及与HBV-DNA复制的关系。方法设正常对照组(n=32)及儿童慢性HBV携带(ASC)组(n=104),ASC组中又根据HBV-DNA的定性分为HBV-DNA阳性(n=62)及阴性组(n=42)。血清HBV标志物和HBV-DNA分别经酶联免疫吸附试验(ELISA)和基因扩增(PCR)法检测,T细胞亚群采用碱性磷酸酶-抗碱性磷酸(APAAP)免疫组化染色法检测。结果ASC组CD3+、CD4+、CD4+/CD8+明显低于对照组,CD8+明显高于对照组(P<0.01);其中HBV-DNA阳性组CD4+、CD4+/CD8+明显低于HBV-DNA阴性组,CD8+明显高于HBV-DNA阴性组(P<0.01或0.05)。结论儿童慢性HBV携带者存在T细胞亚群功能紊乱。其中,尤以HBV-DNA阳性者为著。
AB Objective To investigate the relation between the T-cell subsets and HBVDNA in chronic hepatitis B-virus carrier children Methods 104 chronic hepatitis B-virus carrier children and healthy children（n=32） were selected , the former were divided into HBV-DNA（＋） group （n=62）or HBV-DNA（-） group （n=42） according to HBV-DNA.T-eell subets in the peripheral blood were measured by APAAP ;HBV-signs were measured by ELISA;HBV-DNA were measured by PCR. Results The mean concentrations of CD3＋,CD4＋, CD4＋/CD8＋ of HBV-earrier children were significantly lower than those in control group （P 〈0.01 ）, The level of CDS＋ was markedly higher （P 〈0.01 ）;of them, CD4＋, CD4＋/CD8＋ of HBV-DNA（＋） group were significantly higher than these of HBV-DNA （-） group ,CDS＋ was markedly higher （P 〈0.01 ）;Conclusion There were deficiency of T-cell subsets in HBV-earrier children ,this deficiency were more obvious in HBV-DNA（＋） group.
