从喜温蓝藻分离热稳定限制性内切核酸酶

Isolation of a Thermostable Restriction Endonuclease from Thermophilic Synechococcus Elongatus T3

从喜温蓝藻ＳｙｎｅｃｈｏｃｏｃｕｓｅｌｏｎｇａｔｕｓＴ３分离出一种限制性内切核酸酶，命名为ＳｅｌＩ．此酶是限制性内切核酸酶Ｓａｕ９６Ｉ的同切酶，它们的识别序列ＧＧＮＣＣ．但是ＳｅｌＩ的反应条件与Ｓａｕ９６Ｉ不同．ＳｅｌＩ是一种热稳定的限制性内切核酸酶。

A restriction endonuclease was isolated from thermophilic cyanobacterium Synechococcus elongatus T3, which was named for SelI. This restriction endonuclease was characterized as an isoschizomer of Sau96I, its recognition sequences is GGNCC. But the reaction conditions of SelI are different from that of Sau 96I.Sal I is a thermostable restriction endonuclease with optimal reaction temperature 50￣70℃.