口服ALA在正常小鼠膀胱中的代谢

The Metabolism of ALA Taking Orally in Bladder of Normal Mice

目的:探求光敏剂5-氨基酮戊酸(ALA)口服后在膀胱中的代谢,寻求光敏诊断最佳的ALA口服剂量及时间。方法:予昆明小鼠口服不同剂量的ALA(20mg/kg、40mg/kg、60mg/kg8、0mg/kg 4个组别),不同时间(1小时、2小时、4小时、6小时、8小时、10小时、12小时、24小时、48小时)以激光诱导荧光OMA检测系统测定膀胱卟啉和组织荧光强度,并经数据处理观察ALA不同时间在膀胱中的代谢状态。结果:膀胱卟啉荧光峰值除以组织荧光峰值的比值在口服ALA40 mg/kg后1小时最大。结论:从实验数据分析口服ALA作OMA荧光光谱分析以40mg/kg为最佳检测剂量,最佳检测时间为口服ALA后1小时。

Objective： To research on the metabolism of ALA taking orally in bladder of normal mice and seek suitable dose and diagnostic time of ALA taking orally. Methods： Different dose of ALA taking orally（20mg/kg,40mg/kg ,60mg/kg,80mg/kg）, different time after ALA taking orally（lh,2h,4h,6h,8h,10h,12h,24h,48h）, the fluorescence of bladder and tissue was measured when irradiated by laser. By analyzing the spectrum of fluorescence with optical multi-channel analyzer （OMA） the suitable dose and diagnostic time for tumor detection were obtained. Result：The ratio of Ⅰbladder/Ⅰtissue is largest for 40mg/kg and l h after ALA taking orally. Conclusion：Measuring fluorescence spectrum of bladder and tissue and analyzing the spectrum of fluorescence with optical multi-channel analyzer （OMA）, the most suitable dose was 40mg/kg of ALA taking orally, and the measure time is taken 1 hour after ALA taking orally