长期大量饮酒对大鼠阴茎组织胆碱乙酰转移酶的影响

Effects of long-term and excessive intake of ethanol on choline acetyltransferase activity in the penis of rats

目的探讨长期大量饮酒对大鼠阴茎组织胆碱乙酰转移酶的影响。方法将30只雄性大鼠随机分为对照组、20%酒精剂量组和40%酒精剂量组。分别以生理盐水、20%和40%食用酒精各2mL给大鼠灌胃,每日1次。3个月后,观察各组大鼠性行为和药物诱发阴茎勃起的变化;用化学发光法测定各组大鼠血清睾酮的含量;用免疫组化法测定阴茎平滑肌中胆碱乙酰转移酶(CAT)的含量。结果与对照组相比,20%酒精剂量组大鼠药物诱发阴茎勃起实验没有受到酒精影响(P<0.05),但除射精潜伏期外,酒精抑制了该组大鼠的性行为(P<0.05),而40%酒精剂量组大鼠的性行为表现和药物诱发阴茎勃起实验均受到抑制(P<0.05)。实验组大鼠血清睾酮含量减少(P<0.05),40%酒精剂量组大鼠阴茎组织中CAT活性也降低(P<0.05)。结论大鼠长期大量饮酒后性功能障碍,其机制与血浆睾酮含量降低,阴茎组织中胆碱乙酰转移酶活性降低有关。

Objective To investigate the effects of long-term and excessive intake of ethanol on choline acetyltransferase activity in the penis of rats. Methods Thirty adult male Wistar rats were randomly divided into three groups, the control group, 20 % ethanol group, and 40 % ethanol group. 0.9 % saline （2mL）, 20 % and 40% ethanol solutions（2mL） were administered through gastric tubes in the rats every day. Three months later, sexual behavior and the number of apomorphine-induced penile erection were observed;Testosterone in the sera was determined by chemiluminescence; Choline acetyltransferase activity （CAT） was evaluated by immunohistochemistry. Results Compared with the control group, sexual behavior and testosterone in the sera of the experimental group were inhibited significantly except the ejaculatory latent period of 20% ethanol group （ P 〈 0.05）. The number of apomorphine-induced penile erection and CAT activity in the penis were also decreased notably in 40% ethanol group（ P 〈 0.05）. Conclusion Long-term and excessive intake of ethanol can induce sexual dysfunction in rats. The decline of testosterone in the sera and CAT in the penis may be responsible for it.