毛细管电泳安培检测法同时测定厚朴酚与和厚朴酚

Simultaneous determination of magnolol and honokiol by capillary electrophoresis with amperometric detection

采用毛细管电泳安培检测法对厚朴酚及和厚朴酚的分离测定进行了详细研究.工作电极为0.3 mm碳圆盘电极,30 mmol/L、pH 10.0的硼砂-氢氧化钠为运行缓冲液,分离电压21 kV,检测电位0.95 V.在最优化实验条件下,两待测物在6 min以内完全基线分离.厚朴酚及和厚朴酚的线性范围分别为0.2～50 μg/mL和0.4～60 μg/mL,检测限分别为0.06和0.2 μg/mL.应用于中药厚朴以及中成药霍香正气水和保济丸中厚朴酚及和厚朴酚的检测.

A detailed study on the separation and determination of magnolol and honokiol by capillary electrophoresis with amperometric detection was conducted. The working electrode used was a 0. 3 mm diameter carbon disk electrode. In this work, borax - sodium hydroxide （pH 10. 0, 30 mmol/L） was used as running buffer, 21 kV as the separation voltage and 0. 95 V（ vs. Ag/AgCl, 3mol/L KC1） as the detection potential. Under the optimum conditions, the two analytes were baseline separated within 6 min, Linear range for magolol and honokiol was 0. 2 ～ 50 and 0. 4 ～ 60 μg/mL, respectively. The detection limits were 0. 06 and 0. 2 μg/mL for magnolol and honokiol, respectively. The developed method has been applied to determine magnolol and honokiol in Cortex Magnoliae Officinalis, Huoxiang Zhengqishui and Baojiwan （Chinese medicine） with satisfactory result.