吗啡成瘾大鼠脑神经核、垂体和血浆中内啡肽的变化

Changes of endogenous opioid peptides in neuronucleus,pituitary and plasma in morphine dependent rats

目的：在吗啡成瘾大鼠中观察吗啡成瘾与内啡肽含量变化的关系。方法：用放射免疫法分别测定神经核中β－内啡肽（β－ＥＰ）、亮氨酸脑啡肽（ＬＥＫ）和强啡肽Ａ（ＤｙｎＡ）的含量，垂体和血浆中β－ＥＰ含量。结果：与对照组正常大鼠各指标值相比，成瘾状态大鼠中与依赖和戒断有关的脑神经核——伏隔核（Ａｃｃ）中，β－ＥＰ、ＬＥＫ的水平下降，ＤｙｎＡ的水平上升，血浆、垂体的β－ＥＰ含量均升高。结论：在外源性吗啡作用下，机体这些内啡肽的改变与吗啡的依赖和戒断症状有关。

Objective:To observe the relationship between the changes of the endorphins contents and morphine addiction in the rats with morphine addiction Method:The contents of endorphins in neuronucleus,pituitary and plasma were measured with radioimmunoassay Result:As compared with those in normal rats (as control),in the rats with morphine addiction the contents of β endorphin(β EP),Leucine enkephalin(LEK) decreased significantly (P<0 01)in nucleus accumbens (Acc)that was related to morphine dependence and withdrawal syndroms,whereas the contents of dynorphin A (Dyn A) increased significantly (P<0 01) in Acc;The contents of β EP increased significantly (P<0 01) in pituitary and plasma Conclusion:The changes of endogenous opioid peptides induced by the effects of morphine administration might be related to morphine dependence and withdrawal syndrome