金水宝胶囊清除老年虚证者氧自由基及DNA损伤后修复作用的临床和实验研究

Clinical and Experimental Studies on Elimination of Oxygen Free Radical of Jinshuibao Capsule in Treating Senile Deficiency Syndrome and Its Deoxyribonucleic Acid Damage Repairing Effects

目的：探讨金水宝胶囊清除老年虚证者氧自由基及ＤＮＡ损伤后修复作用。方法：将。股金水宝胶囊者列为治疗组，服淀粉胶囊者列为阴性药物对照组，对老年虚证氧自由基清除及ＤＮＡ损伤后修复作用进行临床和实验研究。结果：治疗前青年组ＳＯＤ活性明显高于老年虚证组，ＭＤＡ含量两组相反（Ｐ＜０．０１）；用药后老年虚证治疗组ＳＯＤ活性升高，ＭＤＡ含量降低（Ｐ＜０．ｏｆ）。对畏寒、头晕、腰膝酸软及夜尿频、耳鸣症状有较好的疗效。动物实验与临床观察一致。老年虚证组治疗前的非程序合成（ＵＤＳ）水平明显低于青年组，而老年虚证治疗组与阴性对照组在用药前后的改变比较差异性有极显著意义（Ｐ＜０．０１）；动物实验表明青年鼠组活体脾脏细胞姐妹染色单体互换（ＳＣＥ）频率明显低于老龄鼠组，金水宝胶囊无诱发ＳＣＥ的作用。结论：金水宝胶囊能提高清除氧自由基及ＤＮＡ损伤后的修复能力。

To observe the efficacy of Jinshuibao capsule. Methods: Senile patients of Deficiency Syndrome treated with Jinshuibao capsule (JSBC) as treated group and with starch capsule as control group. JSBC is a preparation of Cordyceps sinensis. Results: (1) the superoxide dismutase (SOD) activity in senile patient were markedly lower than that in youth, while the malonyldialdehyde (MDA) level of the former was higher than that of the latter, P < 0. 01, (2) The SOD activity increased and the MDA level decreased in the treated group after treatment, P <0.01. JSBC also revealed satisfactory effect on relieving symptoms such as chilling, dizziness , lassitude in loin and legs, frequent nocturia and tinnitus etc. Results of animal experiment wese in accordance with that of clinical observation. The unscheduled deoxyribonucleic synthesis (UDS) level of aged group before treatment was obviously lower than that of youth, after treatment, the change was very significant, and the difference between treated group and control group was also very significant (P <0.01). Animal experiment showed that the sister chromatid exchange (SCE) of splenic cell in young mice group was markedly lower than that in aged mouse group. Conclusion: JSBC has not showed the SCE inducting effect, but could accelerate the repairing of damaged deoxyribonucleic acid (DNA).