抗青蒿酯钠伯氏疟原虫膜表面Con　A结合位点结合量的研究

Study in the Combining Quantity of Con A-Binding Sites on Membrane Surface of Artesunate-Resistance Strain of Plasmodium Berghei

目的：研究疟原虫抗青蒿酯钠的产生机理。方法：以异硫氰酸荧光素－伴刀豆球蛋白Ａ（Ｆ－ＣｏｎＡ）为探针，采用荧光显微分光光度技术，对伯氏疟原虫滋养体膜表面的ＣｏｎＡ结合位点结合量进行了测定。结果：抗青蒿酯钠滋养体膜表面的ＣｏｎＡ结合位点结合量明显低于其正常株。结论：抗青蒿酯钠原虫膜表面的糖类已发生了改变。而膜的流动性变化可能是ＣｏｎＡ结合位点结合量改变的原因之一。

To study the production mechanism of plasmodium berghei (PB) in resisting artesunate. Methods: The combining quantity of Con A-binding sites on membrane surface of PB trophozoite labelled with fluorescein isothiocyanate (FITC) of Con A was determined by fluoromicrospectrophotometer. Results: the combining quantity of PB artesunate-resistance strain (PBAR) was reduced significantly than that of PB nomal strain (P<0.01). Conclusions: The type of glucose in surface membrane of PBAR has changed and the changes of membrane fluidity could be one of the causes of the change in the combining quantity of Con A-binding sites on membrane of PBAR.