牛蒡菊糖脱色工艺的研究

De-coloration technology of burdock oligosaccharide

研究了6种离子交换树脂和吸附树脂对牛蒡菊糖溶液中色素脱除的影响,筛选出3种树脂:非极性大孔吸附树脂DA201-B和DA201-C、大孔弱碱性的阴离子交换树脂D301-G,通过正交试验对脱色条件进行优化。结果表明:采用树脂D301-G,在pH6.5,35℃下,牛蒡菊糖溶液的脱色率可达到95%,多糖保留率为75%,蛋白质去除率87%,牛蒡中的色素可能主要以阴离子色素、非极性小分子色素为主。

De-coloration experiments of burdock oligosaccharides by exchange resins and absorption resins were studied. The effects of six resins on the de-coloration rate of burdock oligosaccharides were investigated. Results show that absorption resins DA201-B and DA201-C, anion exchange resin D301-G represent good de-colorizing abilities. De-colorizing experiments were carried out through orthogonal experiment. The optimum technological parameters are as follows： D301-G resin at 35℃with pH 6.5. The de-coloration rate is 95%, the polysaccharides holding rate is 75%, the protein removal rate is 87%. The results also indicate that burdock colorants are most possibly small-molecular-weight substances with negative charge.