红色荧光和绿色荧光转基因小鼠模型的建立

Establishment of Transgenic Mouse Models Expressing Red and Green Fluorescence

目的建立红色荧光和绿色荧光转基因小鼠,为活体荧光影像系统建立重要的实验动物模型。方法把DsRed-Express和EGFP基因插入chicken-βactin强启动子下游构建转基因载体,建立红色荧光和绿色荧光转基因C57BL/6J小鼠。PCR鉴定红色荧光和绿色荧光转基因小鼠的基因表型,活体荧光影像系统分析红色荧光和绿色荧光转基因小鼠,荧光显微镜检测红色荧光和绿色荧光转基因小鼠全身组织器官的组织形态。结果分别建立了3个系的红色荧光和3个系的绿色荧光转基因小鼠。活体荧光影像系统分析转基因小鼠分别呈现红色荧光和绿色荧光。经荧光显微镜观察,DsRed-Express转基因小鼠的红色荧光蛋白在多个组织器官中表达,尤其在胰腺、肝脏、肾脏和脾脏等器官表达量较高。EGFP转基因小鼠绿色荧光蛋白在全身各个组织器官中表达,尤其在胰腺、心脏、小肠、外周血细胞和脑组织等器官组织中表达量较高。结论DsRed-Express和EGFP基因在转基因小鼠中系统性高表达,成功建立了红色荧光和绿色荧光转基因小鼠。DsRed-Express和EGFP转基因小鼠将成为活体荧光影像系统的重要实验动物模型。

Objective To establish transgenic mouse models exprerssiag DsRed-Express gene and EGFP gene, to be used for in vivo fluorescence imaging analysis. Methods The plasmids were constructed by inserting the DsRed-Express gene and EGFP gene into the down-stream of chicken β-actin powerful promoter. The transgenic mice were genotyped by PCR. The expression of the DsRed-Express gene and EGFP gene were observed under in vivo fluorescence imaging system and fluorescent microscope. Results Three lines of DsRed-Express transgenic C.57BL/6J mice and three lines of EGFP transgenic C57BL/6J mice were established. The red fluorescent protein was expressed in a number of tissues, such as heart, liver, spleen, pancreas, kidney, lung and uterus in the transgenic mouse expressing DsRed-Express. The green fluorescent protein was expressed in almost all tissues in the EGFP transgenic mouse. Conclusions The results of our analysis of red and green fluorescence expression indicated that transgenic mouse models expressing DsRed-Express and EGFP have been successfully established and they may become important animal models for in vivo fluorescence imaging studies.