H5N1型禽流感病毒感染非人灵长类动物的观察

Two Primate Models with Avian Influenza Virus (H5N1) Infection

目的观察H5N1型禽流感病毒对中国非人灵长类动物的易感性并建立动物模型。方法将病毒通过滴鼻接种实验猴,观察感染后动物的临床症状,采血、咽拭子及各器官组织进行血清学、病原学及病理学检查,记录抗体变化、病毒分离情况及病理学改变。结果感染后动物表现轻度食欲下降、一过性体温升高及外周血白细胞减少,肺组织病毒分离及RT-PCR阳性,病理检查感染急性期动物肺组织表现为间质性肺炎,肺泡间隔增宽,充血出血明显,肺泡受压变形,间质及肺泡内有大量炎细胞浸润,符合病毒性肺炎的改变,感染后14 d动物血清IgG抗体水平较感染前升高4倍。结论H5N1病毒可感染非人灵长类动物,可以作为感染模型进行H5N1病毒的发病机制、疫苗评价、药物筛选等研究。

Objective To study the sensitivity of Chinese non-human primates to HSN1 avian influenza virus and to establish animal models for HSN1 research. Method Cynomolgus and rhesus macaques were infected with H5N1 virus through nasal dropping. All monkeys were euthanized at various time points for collecting serum samples for serology, throat swab for etiology and tissues for pathology. Results After infection the animals manifested lack of appetite, transient fever and leukopenia. Virus replication was demonstrated by virus isolation and RT-PCR. Histopathological examination of lung tissues at acute stage confirmed interstitial pneumonia, localized haemorrhage and necrosis, presence of inflammatory infiltration in the interstitium and interalveolar space. On the 14th day after infection, serum IgG level of the macaques went up. Conclusion Chinese rhesus and cynomolgus macaques can be infected with H5N1 virus and may serve as animal models for H5N1 research, providing better insight to the mechanism of AIV infection, and may greatly facilitate the screening of anti-AIV drugs and vaccine evaluation as well.