鲜马鹿茸不同部位多肽的提取及含量比较

Extraction and Comparative Analysis of Polypeptides Contents of Different Segments in Cervus elaphus Linnaeus

采用醋酸溶液和乙醇提取了6支鲜二杠马鹿茸的主干顶部(未骨化部分)、中部(部分骨化)、根部(大部分骨化)3个部位的多肽,进行了多肤的SDS-PAGE分析和胰岛素样生长因子-1(IGF-1)、表皮生长因子(EGF)、神经生长因子(NGF)的Western-blotting分析。采用考马斯亮蓝G-250法进行了马鹿茸不同部位总多肽定量,RIA法测定了IGF-1含量,ELISA法比较了不同部位EGF、NGF的OD值。结果:SDS-PAGE表明鹿茸酸醇提取多肽为一系列分子量低于17kD的混合物,Western-botting证明其含有IGF-1、EGF、NGF等生长因子。定量分析表明:马鹿茸顶部、中部、根部总多肽、IGF-1及醇提多肽中IGF-1含量均差异显著(P<0.05),EGF、NGF ELISA检测OD值差异显著(P<0.05),均从顶部到根部递减。

We extracted the active components of different segments （top, middle ＆ basis） of six fresh velvet antlers-polypeptides with acetic acid solution（pH 3.5）and alcohol and analyzed by SDS-PAGE and Western blotting with anti-human IGF- 1, EGF, NGF as the first antibodies. The total polypeptides and IGF - 1 contents in different segments of velvet antler were detected with Coomassie brilliant blue G - 250 and RIA techniques. EGF and NGF OD values were compared with ELISA. Results： The results of SDS -PAGE indicated that deer velvet antler polypeptides were a mix whose molecular were lower than 17 kD, and Western blotting revealed that IGF - 1, EGF and NGF were extracted with acid and alcohol. Quantitative analysis showed that polypeptides and IGF- 1 extracted with acid and alcohol, and IGF- 1 extracted with acid in different segments of velvet antler were different significantly（ P 〈 0.05）. The OD value difference of EGF and NGF contents between different segments of velvet antler showed by ELISA is also significant（P 〈 0.05）, all the data revealed that they are highly concentrated in the top of velvet antler（ P 〈 0.05）. This test offered an internal quality index in subsection utilization of Cervus elaphus linnaeus.