黄芩苷对小鼠的保胎作用及其对细胞因子的影响

Antiabortive Effect of Baicalin and Its Impact on Cytokines in Mouse

本试验旨在研究黄芩苷的促孕保胎作用及其对母胎界面细胞因子的影响。取怀孕小鼠分成5组,除对照组外,其它4组于孕5-7天皮下注射溴隐亭建立流产模型,孕1-7天分别灌服蒸馏水、0.1、0.51、mg黄芩苷。孕3、5、7、10、12天采样,计算流产率,酶联免疫吸附法(ELISA)测定血清及子宫匀浆中干扰素-γ(IFN-γ)、白介素-10(IL-10)、孕酮的含量。结果孕12天时1 mg黄芩苷组流产率降低,接近对照组,与模型组均有显著差异。孕5天各组血清和子宫匀浆中IFN-γ含量显著上升,其中1 mg黄芩苷组最高。孕酮含量随孕期延长而提高,以0.5和1 mg黄芩苷组最高。表明黄芩苷可剂量依赖性上调孕酮的含量,促进着床期IFN-γ分泌,胚泡附植后又降低IFN-γ的含量,调节着床和妊娠期Th1/Th2细胞因子平衡,影响内分泌-免疫网络,有利于胚胎着床和妊娠维持。

To investigate the effect of Baicalin on abortion model and its impact on cytokines at the maternal-fetal interface,KM mice were divided into 5 groups and all mice except the control were injected with Bromocriptine subcutaneously on days 5 to 7 of pregnancy to establish abortion model. Then the mice in different groups were garaged with distilled water, 0.1, 0.5, 1 mg Baicalin respectively on days 1 to 7 of gestation while the control group treated with ethanol and distilled water. Samples were collected on day 3, 5, 7, 10 and 12 to calculate abortion rate. The content of interferon （IFN）-γ, interleukin （IL）-10, progesterone in serum and uterine supernatant were measured by Enzyme-Linked Immuno-absorbent Assay （ELISA）. Results showed that the abortion rate in 1 mg Baicalin group at day 12 of gestation was similar to that of control group, which was significantly different from all the abortion models. The volume of IFN-γ was significantly increased in all groups especially in lmg Baicalin group throughout the implantation stage （day 5 of gestation）. As the gestation progresses, the content of progesterone increased gradually with the significant higher level in 0.5 mg and 1 mg Baicalin groups. These results indicate that Baicalin up-regulates the level of progesterone in a dose-dependent manner, enhances the secretion of IFN-γ during implantation and down-regulates the IFN-γ production afterwards. These findings suggest that Baicalin can modulate cytokine network in pregnancy and benefit embryo implantation and survival.