雷公藤甲素对大鼠肺切除加野百合碱诱导肺动脉高压的影响

Effect of Triptolide on the Development of Monocrotaline-induced Pulmonary Hypertension in Pneumonectomized Rat

目的观察雷公藤甲素(TL)对大鼠左肺切除加野百合碱所致肺动脉高压和右心室肥厚的影响作用。方法将SD雄性大鼠随机分为正常组、模型组、TL连续治疗组、安慰剂1组、TL延迟治疗组和安慰剂2组。采用光镜、图像分析和免疫组织化学方法,观察各组大鼠肺动脉平均压,右心室/(左心室+室间隔)质量比,肺小动脉管壁百分比,肺小动脉无肌性动脉肌化程度,新生内膜所致血管阻塞计分的变化。结果①模型组各指标均高于正常组(P<0.01)。②两治疗组各指标均小于相应两安慰剂组和模型组(P<0.05)。③两治疗组之间除肺小动脉无肌性动脉肌化程度外其余各指标比较差异有统计学意义(P<0.05)。结论雷公藤甲素对肺切除加野百合碱所致肺动脉高压和右心室肥大有逆转作用,且对肺动脉高压形成前和形成后均有作用。

Objective To investigate the effect of Triptolide on the development of monocrotaline（MCT）-induced pulmonary hypertension and right ventricular hypertrophy in pneumonectomized rat.Methods Sixty male Sprague-Dawley rats were randomly divided into continuous Triptolide therapy group,delayed Triptolide therapy group,two placebo groups,model group and normal group,of which the mean pulmonary arterial pressure（mPAP）,right ventricular index（RV/LV＋S） were observed or checked.The light microscope, image analysis and immunohistochemistry were used to show percent vascular wall thickness（WT%）,the degree of muscularization and vascular occlusion score in pulmonary arteries.Results ① Each index of model group was obviously increased（P〈0.01 vs common group）.② The indexes of two therapy groups were attenuated for the pneumonectomized rats that suffered from MCT induced pulmonary hypertension（P〈0.05 vs model group and placebo group）.③ There was the statistics significance between the two therapy groups in all indexes except for the degree of muscularization（P〈0.05）.Conclusion For pneumonectomized rats that suffer from the MCT induced pulmonary hypertension and undergo the pulmonary vascular remodeling,triptolide can slow down pulmonary hypertension,right ventricular hypertrophy and promote the regression of pulmonary arterial neointimal formation before and after forming pulmonary hypertension.