细胞保护蛋白在人体移植肾中的表达

Expressions of Cytoprotective Proteins for Human Renal Allografts

目的研究细胞保护蛋白在人体移植肾中的表达情况和临床意义。方法采用免疫组化染色技术检测人体移植肾急性排斥反应(AR)、慢性排斥反应(CR)和无排斥反应(NR)3组各10例中细胞保护蛋白A20、血红素加氧酶-1(HO-1)和B细胞淋巴瘤/白血病-XL蛋白(Bcl-XL)的表达情况。结果A20、HO-1和Bcl-XL主要表达于内皮细胞、平滑肌细胞和浸润性单核细胞。A20在AR中表达强于CR〔(5.04±0.71)vs(3.20±0.64),P<0.01〕;A20在NR中不表达。HO-1在AR中表达(7.91±2.24),在CR和NR中不表达。Bcl-XL在3组中均表达(AR:10.62±3.17;CR:8.50±2.45;NR:11.03±2.77),但在CR中表达较弱;AR、NR与CR相比,差异均具有统计学意义(P<0.05);而AR组和NR组间的差异无统计学意义。结论A20和HO-1可能在AR中发挥细胞保护作用;而在CR中,A20可能是发挥保护作用的主要蛋白。

Objective To investigate the expression status and clinical significance of cytoprotective proteins to human renal allografts.Methods Expressions of cytoprotective proteins： antiapoptotic protein（A20）,heme-oxygenase-1（HO-1）,and B-cell lymphoma/leukemia-XL（Bcl-XL） were analyzed by immunohistochemistry and compared in 30 renal allografts,including 10 grafts undergoing acute rejection（AR）,10 grafts undergoing chronic rejection（CR）,and 10 nonrejecting（NR） grafts.Results Expressions of A20,HO-1 and Bcl-XL localized mainly in endothelial,smooth muscle,and infiltrating mononuclear cells.A20 expressed in grafts undergoing AR（5.04±0.71） and CR（3.20±0.64）,not in NR grafts,and its expression in CR was weaker than that in AR（P〈0.01）.HO-1 expressed in AR（7.91±2.24）,not in CR and NR.Bcl-XL was detected in all grafts（AR： 10.62±3.17;CR： 8.50±2.45;NR： 11.03±2.77）,but had a decreased expression levels in CR.Conclusion A20 and HO-1 may play protective role for AR,and A20 may be the essential protein having protective function for CR.