沙利度胺对子宫内膜异位症的内膜间质细胞体外表达VEGF的影响

Effects of thalidomide on vascular endothelial growth factors expression in endometrial stromal cells of endometriosis in vitro

目的:观察沙利度胺(ThD)对子宫内膜异位症病人内膜间质细胞的增殖及其血管内皮生长因子(VEGF)表达的影响。方法:刮取子宫内膜异位症病人的子宫内膜组织,经体外分离、培养,用波形蛋白单抗进行免疫组化染色鉴定,应用四唑盐(MTS)分析肿瘤坏死因子α(TNF-α)和ThD对间质细胞生长的影响,以实时荧光定量PCR、Western Blot和定量ELISA分析在存在外源TNF-α的情况下,不同浓度ThD对间质细胞表达VEGF的影响。结果:TNF-α能促进子宫内膜间质细胞的增殖和分泌VEGF,而ThD能剂量依赖性抑制内膜间质细胞的增殖,并逆转TNF-α对VEGF的上调作用。结论:ThD可抑制体外培养的子宫内膜异位症内膜间质细胞的增殖及其VEGF的表达和分泌,提示ThD对于治疗子宫内膜异位症可能具有一定的作用。

AIM ： To observe the effects of thalidomide （ThD） on cell proliferation and vascular endothelial growth factors （VEGF） expression in endometrial stromal cell of endometriosis in vitro. METHODS： Human endometrial tissue was obtained from patients with endometriosis. The stromal cells were isolated and identified by immunohistochemical staining with vimentin antibody. The influence of tumor necrosis factor-alpha （TNF-α） and ThD on proliferation of endometrial stromal cells was observed. The effects of different concentration of ThD on VEGF expression in endometrial stromal cells were analyzed by real-time PCR, Western Blot and ELISA. RESULTS： TNF-α could promote the proliferation of endometrial stromal cell and the secretion of VEGF, while ThD could inhibit the proliferation of endometrial stromal cell and reverse the up-regulation of VEGF expression by TNF-α. CONCLUSION： ThD could inhibit the proliferation of endometrial stromal cell and expression and secretion of VEGF in vitro, which suggested that ThD might have therapeutic effect on endometriosis.