伊曲康唑和γ-干扰素对裸小鼠、昆明种小鼠外周血IFN-γ、IL-10的影响

Effect of Itraconazole/IFN-gamma on IFN-gamma and IL-10 in Nude Mice and Mice

目的研究伊曲康唑和γ-干扰素治疗分别感染白念珠菌和新生隐球菌裸小鼠、昆明种小鼠外周血细胞因子IFN-γ、IL-10的改变及其临床意义。方法将白念珠菌和新生隐球菌分别注射入裸小鼠、昆明种小鼠体内,诱导成动物模型。用ELISA法检测IFN-γ和IL-10的水平。结果经过伊曲康唑和γ-干扰素治疗后,其外周血的IFN-γ上升而IL-10下降。结论伊曲康唑和γ-干扰素对真菌感染小鼠免疫功能的调节起着重要作用。

Objective To study the effect of itraconazole/IFN - gamma on IFN - γ and IL - 10 in nude mice and mice and clinical significance. Methods Systemic infection of animals that induced by abdominal injection of Candida albicans and Cryptococcus neoformans, Expression of IFN - γ and IL - 10 were detected by enzyme - linked immunosorbent assay （ ELISA）. Results After treated with Itraconazole and IFN - gamma, the levels of IFN - gamma were increased and ones of IL - 10 were decreased significantly. Conclusions Itraconazole and IFN - gamma may play key roles in the immune function of those animals.