犬黑素皮质素受体-2基因的分子克隆及序列分析

Molecular Cloning and Sequence Analysis of Melanocortin 2-Receptor Gene in the Dog

目的分离犬MC2R基因cDNA5′末端,分析其启动区域特点。方法采用了RNA连接酶介导的RACE(RLM-RACE)技术分离了犬MC2R基因和局部序列比对工具(Basic Local Alignment Search Tool,BLAST)对CDS区进行了初步验证。结果新分离了犬MC2RcDNA的5′末端,并对其启动区序列作了初步分析。序列分析显示,该基因至少由两个外显子(exon1和exon2)组成,exon1和exon2的一部分编码5′非翻译区(5′-UTR),exon2其余的部分编码整个编码区。结论克隆了犬MC2R基因的5′末端,在其启动区发现了inr、SF-1、SP1、CRE、PPRE、AP-1等多个顺式作用元件,为犬MC2R表达调控研究奠定基础。

Objective To isolate the 5＇-UTR of MC2R gene in dog and analyze its promoter region. Methods RLMRACE and BLAST analysis tool were used in this research. Results The 5＇-UTR were sequenced with a length of 168 bp. There was a 88% identity between the sequence result and that reported in canis, human and mouse, respectively. Two exons and the lengths of each exon and intron were predicted. Several kinds of cis-acting elements and its amounts have been analyzed. Conclusions MC2R gene is expressed in adrenal gland of dogs, and its promoter region is made up of several kinds of cis-acting elements.