43株念珠菌分离鉴定和药敏试验研究

Identification and Drug Susceptibility Test of 43 Clinical Isolation of Candida Strains

[目的]了解皮肤病、性病门诊就诊者念珠菌感染状况及其对抗真菌药物敏感性,为该菌的防治提供依据。[方法]用VITEK32细菌鉴定仪对念珠菌进行鉴定,用MTT法进行药物敏感性试验。[结果]从85份临床标本分离到43株念珠菌,阳性分离率为50.6%。其中白念珠菌30株(69.8%)、近平滑念珠菌6株(14.0%)、光滑念珠菌4株(9.3%)、季也蒙念珠菌2株(4.7%)、丛生丝孢酵母菌1株(2.3%)。MTT法测定念珠菌对氟康唑和伊曲康唑的总敏感率分别为93.0%和67.4%,与常规法比较差异无统计学(P>0.05)。[结论]白念珠菌仍是最常见的致病念珠菌。念珠菌对氟康唑的总敏感率略高于伊曲康唑,对氟康唑耐药的菌株往往对伊曲康唑也耐药。

[Objective]To understand the infection and drug susceptibility status of candida among clinical patients t provide scientific basis for prevention and control. [Methods]All candida isolated were identified by VITEK 32 Microbe Identification System. MTT assay was used for the fluconazole and itraconazole susceptibility. [Results]43 candida were isolated from 85 clinical specimens and the positive rate was 50.6%. C. albicans accounted for 30 （69.8%） followed by C. parapsilosis 6 （14.0%） ,C. glabrata 4 （9.3%） ,C. guillermondii 2 （4.7%）, and trichosporon pullulans 1 （2.3%）. The total susceptibility rate of candida to fluconazole was 93.0%, and to itraconazole was 67.4%. There was not difference compared to routine clinical method. [Conclusion] The most common species in the 43 clinical isolates were still C. albicans. The total susceptibility rate to fluconazole was higher than itraconazole. Some of the candida were resistant to fluconazole which were also cross-resistant to itraconazole.