8个亚洲水牛群体的遗传结构分析

Analysis on the genetic structure of 8 Asia buffalo populations

应用13个微卫星标记结合荧光–多重PCR技术,对德昌水牛、兴隆水牛、富钟水牛、温州水牛、东流水牛、福安水牛和两个引进品种摩拉水牛、尼里-拉菲水牛进行遗传结构分析。结果表明:8个水牛群体在13个微卫星座位中共检测到157个等位基因,其中7个群体具有各自的特有等位基因,其和为23;8个群体的有效等位基因数在2.2908～4.2308之间,杂合度在0.4951～0.7194之间,多态信息含量在0.4495～0.6776之间;有11个座位为高度多态座位,是适合分析水牛遗传多样性的多态标记;聚类分析表明富钟水牛和东流水牛先聚在一起,再与兴隆水牛聚在一起,然后与温州水牛和福安水牛聚在一起,德昌水牛独自聚为一类;两个引进品种聚为一类。

One hundred and forty seven alleles were detected when thirteen microsatellite loci were analyzed applying fluorescence-multiplex PCR technology in eight buffalo populations were analyzed, including six indigenous Chinese native breeds （Dechang. Xinglong. Fuzhong. Wenzhou. Dongliu. Fu＇an）, and two introduced breeds （Murrah. Nili-Ravi）. Seven populations have their own unique alleles, total number is twenty-three. As to all the eight populations, effective number of alleles （Ne） was between 2.2908 and 4.2308, heterozygosity （H） between 0.4951 and 0.7194, and polymorphism information content （PIC） between 0.4495 and 0.6776. Eleven of the thirteen microsatellite loci were of high polymorphism and were then the appropriate, polymorphism marker could be used to analyze properly genetic diversity of the involved buffalo populations. Cluster analysis indicated that Fuzhong and Dongliu were clustered together, then with an independent cluster of Xinglong. Wenzhou and Fu＇an were clustered together, Dechang was an independent cluster. Murrah and Nili-Ravi were clustered together.