应用重组自交系群体定位大豆抗虫QTL

Mapping insect resistance QTLs of soybean with RIL population

以大豆组合科丰1号×南农1138-2衍生的重组自交系(RIL)群体为材料构建遗传连锁图谱,利用软件Cartographer V.2.5采用复合区间作图法检测定位大豆抗虫QTL。以斜纹夜蛾幼虫重为抗性指标,检测到1个与抗虫性有关的QTL,位于G20-O连锁群上,其端距离为31.91cM,加性效应估计值为0.0408,对性状变异的解释率为11.74%;以蛹重为抗性指标,检测到2个与抗虫性有关的QTL,分别位于G8-D1b+W和G17-L连锁群上,其端距离分别为14.71cM和0.01cM,加性效应估计值分别为-0.0139和0.0103,对性状变异的解释率分别为11.30%和6.36%。

A recombinant inbred line （RIL） population, NJRIKY, which was derived from the cross Kefeng 1 xNannong 1138-2, was used to constructed the genetic linkage map. Larval weight and pupae weight of cotton worm [Prodenia litura （L.） Fabricius] were examined and used as indicators of resistance. Based on the linkage map constructed with SSR markers of this RIL population, one QTL responsible for larval weight was mapped on linkage group G20-O and the position was 31.91 cM. The QTL＇s additive effect was 0.0408 and explained 11.74% of the total variation of the larval weight. Two QTLs associated with pupae weight were mapped on linkage group G8-D1b＋W and G17-L and the positions were 14.71 cM and 0.01 cM, respectively. The QTLs＇ additive effects were -0.0139 and 0.0103 ,which explained 11.30% and 6.36% of the total variation of larval weight, respectively.