茶多酚联合反应停对人肺腺癌A549抑制作用研究

Study on Inhibitory Effect of Tea Polyphenols Combined with Thalidomide on Human Lung Adenocarcinoma A549

目的:观察茶多酚联合反应停对人肺腺癌生长的抑制作用;为研究茶多酚抗肿瘤血管生成提供依据。方法:人源肺腺癌(A549)细胞系经传代培养后,以BALB/c-nu裸鼠进行肿瘤移植,并以茶多酚,反应停及其联合用药进行干预性治疗,通过观察抑瘤率评价单用及联合用药对A549移植瘤生长抑制作用。结果:茶多酚、反应停及联合用药组的抑瘤率分别为33%、21.6%和35.9%,茶多酚、联合用药组与模型组相比有统计学意义(P<0.05);联合用药组与单用药组比,无统计学意义(P>0.05)。结论:茶多酚对A549生长有明显的抑制效果;反应停对A549的抑制作用需进一步观察;两者联合使用未见明显增效作用。

Objective： To observe the inhibitory effect of tea polyphenols（TPP） combined with thalidomide on human lung adenocarcinoma（HLAC） and to provide basis for studying TPP against tumor angiogenesis. Methods： After serial subcultivation, HLAC（A549 ） was implanted on BALB/c nude mice（n=32）. The 32 nude mice were divided into model control group, TPP group, Thalidomide group, and TPP plus thalidomide group. The model group was given physiological saline; TPP, thalidomide, and TPP plus thalidomide were given to the TPP group, thalidomide group and the combined medication group for intervention therapy. The inhibitory effects of the single drug and combined drugs on the growth of A549 implanted tumor were evaluated by the observation of tumor inhibitory rate. Results： The tumor inhibitory rate of TPP, thalidomide and the combined medication groups was 33%, 21.6% and 35.9%. Compared with the model control group, there were statistical differences in TPP group and the combined medication group（p〈0.05 ）; while there was no statistical difference between the combined medication group and single drug group（p〉0.05 ）. Conclusion： Statistically, the inhibitory effect of the combined medication on implanted HLAC is not superior to the single drug, but its tumor inhibitory rate is higher than that of the single drug, which needs to be further studied in the clinical practice in future.