甲基蓝共振瑞利散射法测定穿琥宁

Resonance Rayleigh Scattering Method for the Determination of Patassium Dehydroandrographolide Succinate in its Injection Solution with Methyl Blue

目的通过实验,以期建立一种测定穿琥宁的新方法。方法在pH4.4的酸性溶液中,取适量对照品溶液和甲基蓝溶液混合,以二次蒸馏水稀释,放置一段时间后在荧光光度计上以λem=λex方式进行同步扫描。结果甲基蓝和穿琥宁能够迅速反应生成离子缔合物,导致共振瑞利散射(RRS)光谱的改变,产生比较特征的新的RRS光谱,最大峰值在650.0nm,并且在2.0-14.0×10-5mol/L范围内,RRS强度和穿琥宁的浓度成正比。结论我们建立了测定穿琥宁的共振瑞利散射法。该法灵敏度较高,检测限达到2.65×10-5mg/L,用于检测某些穿琥宁样品中穿琥宁的含量,结果较满意。

Objective We wish to find a method to determine PDS in medicine.Methods In pH 4.4 HAc-NaAc butter solution,we mixed patassium dehydroandrographolide succinate（PDS）,water and Methyl blue（MB） with proper properation.After a while,we put it in Hitachi F-2500 and determined the resonance Rayleigh scattering（RRS） spectra.Results MB reacts rapidly with PDS to form complexes,which resulted in great enhancement of RRS and a new RRS spectrum.The maximum RRS peak was near 650.0nm.RRS intensity is proportional to the concentration of PDS in the range of 2.0-14.0×10-5mol/L.Conclusion We found a method to determine PDS based on our experiments.The method has high sensitivity and the detect limit（3σ） for PDS is 2.65×10^-5mg/L.The method has been used for the determination of total amounts of PDS in samples with satisfactory results.