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Expression of Survivin and Its Splice Variants in Gastric Cancer 被引量：1

Expression of Survivin and Its Splice Variants in Gastric Cancer
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摘要 Survivin variants specific real time quantitative RT-PCR was developed to analyze their expression in 53 paired cancer and para-cancerous tissues, and the expression of the wild-type survivin protein was detected by immunohistochemistry. The results showed that survivin mRNA and protein were expressed in gastric cancer and para-cancerous tissues, The survivin-2B was dominantly expressed in para-cancerous tissues, whereas the survivin-△Ex3 was more frequently detected in cancer tissues. The positive rate of survivin-2a was 100% in both cancer and para-cancerous tissues, but its relative transcript expression level was not significantly increased in cancer tissues in comparison with para-cancerous tissues. The correlation analysis revealed that the expression of survivin-2a mRNA was significantly associated with that of total survivin （rs=0.4178, P=0.0018）, whereas inversely to that of survivin-△EX3 （rs=-0.4506, P=0.0007）. It was suggested that survivin-2a may act as an antagonist of survivin-△EX3. The balance between antiapoptotic survivin iso-forms and nonantiapoptotic ones may play an important role in tumorigenesis and tumor progression, Promising value is hinted to analyze survivin and its variants in tumor early diagnosis and distinguishing malignant tumors from benign ones. Survivin variants specific real time quantitative RT-PCR was developed to analyze their expression in 53 paired cancer and para-cancerous tissues, and the expression of the wild-type survivin protein was detected by immunohistochemistry. The results showed that survivin mRNA and protein were expressed in gastric cancer and para-cancerous tissues, The survivin-2B was dominantly expressed in para-cancerous tissues, whereas the survivin-△Ex3 was more frequently detected in cancer tissues. The positive rate of survivin-2a was 100% in both cancer and para-cancerous tissues, but its relative transcript expression level was not significantly increased in cancer tissues in comparison with para-cancerous tissues. The correlation analysis revealed that the expression of survivin-2a mRNA was significantly associated with that of total survivin （rs=0.4178, P=0.0018）, whereas inversely to that of survivin-△EX3 （rs=-0.4506, P=0.0007）. It was suggested that survivin-2a may act as an antagonist of survivin-△EX3. The balance between antiapoptotic survivin iso-forms and nonantiapoptotic ones may play an important role in tumorigenesis and tumor progression, Promising value is hinted to analyze survivin and its variants in tumor early diagnosis and distinguishing malignant tumors from benign ones.
作者 程正江 胡丽华 付文荣 张勤 廖晓峰
机构地区 Department of Clinical Laboratory Medicine Department of Pathology Department of General Surgery
出处 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2007年第4期393-398,共6页 华中科技大学学报（医学英德文版）
关键词 SURVIVIN splice variants real-time PCR survivin splice variants real-time PCR
分类号 R735.2 [医药卫生—肿瘤]
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参考文献10

  • 1Altieri D C,Marchisio C.Survivin apoptosis: An inter- loper between cell death and cell proliferation in cancer[].Lab Invest.1999
  • 2Mahotka C,Wenzel M,Springer E et al.Survivin- del- taEx3 and survivin-2B: two novel splice variants of the apoptosis inhibitor survivin with different antiapoptotic properties[].Cancer Research.1999
  • 3Krieg A,Mahotka C,Krieg T et al.Expression of differ- ent survivin variants in gastric carcinomas: first clues to a role of survivin-2B in tumor progression[].British Journal of Cancer.2002
  • 4Tellmann G.The E-Method: a highly accurate technique for gene-expression analysis[].Nature Methods.2006
  • 5Span P N,Tjan-Heijnen V C,Heuvel J J et al.Do the survivin (BIRC5) splice variants modulate or add to the prognostic value of total survivin in breast cancer?[].Clin Chem.2006
  • 6Chiou S K,Moon W S,Jones M K et al.Survivin expres- sion in the stomach: implication for mucosal integrity and protection[].Biochemical and Biophysical Research Communications.2003
  • 7Noton E A,Colnaghi R,Tate S et al.Molecular analysis of survivin isoforms: evidence that alternatively spliced variants do not play a role in mitosis[].Journal of Biological Chemistry.2006
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  • 9Islam A,Kageyama H,Takada N et al.High expression of survivin,mapped to 17q25,is significantly associated with poor prognostic factors and promotes cell survival in human neuroblastoma[].Oncegene.2000
  • 10Ambrosini G,Adida C,Altieri DC A novel anti-apoptosis gene,survivin.expressed in cancer and lymphoma[].Nature Medicine.1997

同被引文献10

  • 1Obiol-Pardo C,,Granadino-Roldan JM,Rubio-Martinez J.Protein-protein recognition as a first step towards the inhibition of XIAP and Survivin anti-apoptotic proteins[].Journal of Molecular Recognition.2008
  • 2LiuQ,Fu H,Xing R,Tie Y,Zhu J,Sun Z,Zheng X.Survivin knock-down combined with apoptin overexpression inhibits cell growth significantly[].Cancer Biology and Therapy.2008
  • 3Kishi H,Igawa M,Kikuno N,Yoshino T,Urakami S,Shiina H.Expression of the Survivin gene: correlation with clinicopathological characteristics, proliferativity and apoptosis[].Journal d Urologie.2004
  • 4Cheng Z,Hu L,Fu W,Zhang Q,Liao X.Expression of Survivin and its splice variants in gastric cancer[].Huazhong Univ Sci Technolog Med Sci.2007
  • 5Komuro Y,Watanabe T,Tsurita G,Muto T,Nagawa H.Survivin expression in ovarian carcinoma: correlation with apoptotic marker and prognosis[].Modern Pathology.2003
  • 6Zhang R,Wang T,Li KN,Qin WW,Chen R,Wang K, et al.A Survivin double point mutant has potent inhibitory effect on the growth of hepatocellular cancer cells[].Cancer Biology and Therapy.2008
  • 7Oshita F,Ito H,Ikehara M,Ohgane N,Hamanaka N,Nakayama H, et al.Prognostic impact of Survivin, cyclin D1, integrin[beta]1, and VEGF in patients with small adenocarcinoma of stage I lung cancer[].American Journal of Clinical Oncology.2004
  • 8Pennati M,Folini M,Zaffaroni N.Targeting Survivin in cancer therapy[].Expert Opin TherReview.2008
  • 9Gonzalez S,Aubert S,Kerdraon O,Haddad O,Fantoni JC,Biserte J, et al.Prognostic value of combined p53 and Survivin in pT1G3 urothelial carcinoma of the bladder[].American Journal of Clinical Pathology.2008
  • 10Altieri DC,Clynes M,Goden G,et al.Survivin and apoptosis control[].Advances in Cancer Research.2003

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Journal of Huazhong University of Science and Technology(Medical Sciences)
2007年 第4期

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