摘要
目的研究僵蚕抗凝血酶诱导血管内皮细胞释放作用的影响。方法将不同浓度的僵蚕注射液(600μg/mL,300μg/mL,150μg/mL)加入凝血酶诱导的血管内皮细胞中,培养12h后收集细胞液,观察不同浓度的僵蚕注射液对凝血酶诱导血管内皮细胞释放作用的影响。结果僵蚕大、中、小剂量组较凝血酶组均能明显增加组织型纤溶酶原激活物(t-PA)活性,分别为(0.460±0.010)U/mL,(0.457±0.019)U/mL,(0.366±0.014)U/mL(P<0.01);同时也可降低纤溶酶原活化素抑制物(PAI)活性,降低血管性假血友病因子(vWF)含量,与凝血酶组比较差异具有统计学意义(P<0.01)。结论僵蚕注射液可以明显抑制凝血酶诱导的内皮细胞释放作用,此作用与僵蚕的抗凝作用有关。
Objective To study the effect of bombyx botrytieatus on vascular endothelial cell induced by thrombin. Methods Three different doses of bombyx batryticatus injection were added to vascular endothelial cell induced by thrombin, After 12 hours, the cell ,sap wacollected and the effect of different concentration bombyx batryticaus on vascular endothelial cell induced by thrombin was investigated. Resuits Bombyx botrytieatus could increase the activity of t - PA, decrease PAI activity and vWF content. Conclusion Bombyx botryticatus could inhibit the release of vascular endothelial cell induced by thrombin, which related to the anticoagulation of bombyx botryticatus.
出处
《中西医结合心脑血管病杂志》
2007年第9期837-839,共3页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
关键词
僵蚕注射液
血管内皮细胞
凝血酶
bombyx Botryticatus
vascular endothelial cell
thrombin
