城市污水SBR法短程生物脱氮系统硝化菌群的定量分析

Quantitative Analysis of Nitrifying Communities in Short-cut Nitrogen Removal From Municipal Wastewater of SBR

为了研究实时控制对短程生物脱氮中试系统内硝化菌群结构组成的影响,在考察系统常温条件下处理城市污水时短程硝化效果的同时,采用FISH、PCR-DGGE和PCR-克隆序列(Cloning Sequencing)分子生物学方法对SBR中试系统(有效容积为54 m^3)硝化菌群中的氨氧化菌(AOB)和亚硝酸氧化菌(NOB)进行定性与定量化分析.FISH结果表明,在短程脱氮系统中,AOB相比于NOB已成为明显的优势菌群,占总菌群的3%～12%;且没有检测出NOB.PCR-DGGE结果表明,SBR短程脱氮中试系统中的AOB均以Nitrosomonas-like为主.污泥样品的PCR-Cloning-Sequencing结果表明,所有的克隆相似于Nitrosomonas,其中60%以上的克隆相似于Nitro- somonas europaea.

To investigate the effects of real-time control on the structure of nitrifying communities of the short-cut nitrogen removal in SBR pilot-scale plant with a working volume of 54 m^3, molecular techniques of Fish, PCR-DGGE and PCR-Cloning-Sequencing were used to analyze ammonia-oxidizing bacteria （AOB） and nitrite-oxidizing bacteria （NOB） with the performance of short-cut nitrogen removal. Fish results show that in short-cut nitrogen removal process, AOB became dominant with compared to NOB and accounted for 3 % 12% of the total biomass. More importantly, no NOB was detected. PCR-DGGE gel results show that AOB were phylogenetically related to Nitrosomonas-like species with a melting point at the range between 30 % to 50%. PCR-Cloning-Sequencing results show that all clones were affiliated with Nitrosomonas corresponding to DGGE results and 60% of the clones were affiliated with Nitrosomonas europaea.