SHH、EGFR和PCNA蛋白在胰腺癌组织中的表达及其意义

Expression of Sonic Hedgehog, EGFR and PCNA Proteins in Pancreatic Cancer and Their Correlations to Cell Proliferation

背景与目的:SHH(sonic hedgehog)信号通路为胚胎发育过程中调节细胞间相互作用的重要信号通路,表皮生长因子受体(epidermal growth factor receptor,EGFR)具有调节细胞增殖、分化的作用,增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)是反映细胞增殖的指标。本研究的目的是探讨SHH、EGFR和PCNA蛋白在胰腺癌组织中的表达及其意义。方法:采用免疫组化方法检测49例胰腺癌组织及癌旁组织中SHH、EGFR蛋白和PCNA的表达情况,并分析其相关性。结果:SHH和EGFR蛋白在胰腺癌组织中的阳性率分别为79.6%和73.5%,与癌旁组织(14.3%和16.3%)相比,差异有统计学意义(P<0.05)。SHH和EGFR蛋白表达与患者年龄、肿瘤大小、组织学类型和肿瘤部位等均无关(P>0.05),而与淋巴结转移状况和TNM分期有关(P<0.05)。胰腺癌组织中83.7%呈PCNA蛋白高表达,与癌旁组织(20.4%)相比,差异有统计学意义(P<0.05)。Spearman相关分析显示,SHH与EGFR蛋白表达呈正相关关系(r1=0.232,P<0.05)。PCNA蛋白表达与SHH和EGFR蛋白表达均呈正相关性(r2=0.412,P<0.05;r3=0.186,P<0.05)。结论:SHH和EGFR信号通路在胰腺癌组织中均呈激活状态,并且与胰腺癌细胞的增殖活性相关。

BACKGROUND ＆ OBJECTIVE： Sonic hedgehog （SHH） signaling pathway is important for modulating cellular interaction in the development of mammalian embryo. Epidermal growth factor receptor （EGFR） has been involved in the regulation of cell proliferation and differentiation. Proliferating cell nuclear antigen （PCNA） is an indicator of cell proliferation. This study was to investigate the expression of SHH, EGFR and PCNA in pancreatic cancer, and explore their correlations to cell proliferation. METHODS： The expression of SHH, EGFR, and PCNA in 49 specimens of pancreatic cancer and 49 specimens of normal pancreatic tissues adjacent to cancer was detected by immunohistochemistry. Their interrelations were analyzed. RESULTS： The positive rates of SHH and EGFR were significantly higher in pancreatic cancer than in normal tissue adjacent to cancer （79.6% vs.14.3%, 73.5% vs.16.3%, P〈0.05）. The expression of SHH and EGFR in pancreatic cancer had no correlation to patients＇ age, tumor size, pathologic type, and tumor site （P〉0.05）, but were correlated to lymph node metastasis and TNM stage （P〈0.05）. The strong positive rate of PCNA was significantly higher in pancreatic cancer than in normal tissues （83.7% vs. 20.4%, P〈0.05）. SHH expression was positively correlated to EGFR expression （r1=0.232, P〈0.05）. SHH and EGFR expression were positively correlated to PCNA expression（r2=0.412, P〈0.05; r3=0.186, P〈0.05）. CONCLUSION： SHH and EGFR signal pathways are active in pancreatic carcinoma, and are closely correlated to cancer cell proliferation.