转录因子Sp1在乳腺癌组织中的表达及其预后意义

Expression and Prognostic Value of Transcriptional Factor Sp1 in Breast Cancer

背景与目的:已知转录因子Sp1参与几乎所有的细胞功能,包括细胞增殖、凋亡、分化和新生物的转化,在恶性肿瘤侵袭与转移中发挥重要作用,但不同来源肿瘤的Sp1表达也不尽相同。本研究旨在探讨Sp1在乳腺癌组织中的表达及其与乳腺癌侵袭、转移及预后的关系。方法:用免疫组化EnVision法检测60例乳腺癌及12例癌旁乳腺组织中Sp1的表达情况。结果:Sp1在乳腺癌组织中的阳性率为71.67%(43/60),在癌旁乳腺组织中的阳性率为33.33%(4/12)。乳腺癌组织中Sp1表达与乳腺癌患者TNM分期呈正相关(r=0.349,P<0.05),与癌组织浸润呈正相关(r=0.407,P<0.01),与淋巴结转移呈正相关(r=0.314,P<0.05)。单因素生存分析显示乳腺癌组织Sp1阳性组的总生存率低于阴性组(分别为41.86%和82.35%,P<0.05)。Cox模型多因素分析显示乳腺癌组织Sp1表达、TNM分期、癌组织浸润及淋巴结转移是影响乳腺癌患者预后的独立因素。结论:Sp1可能参与了乳腺癌的侵袭与转移,是乳腺癌预后不良因素之一;检测Sp1的表达并结合肿瘤浸润和临床分期分析能提高对乳腺癌患者预后判断的准确性。

BACKGROUND ＆ OBJECTIVE： Transcriptional factor Sp1 is involved in many biological processes, such as cell proliferation, apoptosis, differentiation and transformation, and plays an important role in invasion and metastasis of tumors. However, the expression patterns of Sp1 in various tumors are different. This study was to investigate the correlations of Sp1 expression to metastasis, invasion, and prognosis of breast cancer. METHODS： The expression of Sp1 in 60 specimens of breast cancer and 12 specimens of adjacent breast tissue was detected by EnVision immunohistochemistry. Its correlation to clinicopathologic features of breast cancer was analyzed by Cox regression analysis. RESULTS ： The positive rate of Sp1 was 71.67% in breast cancer tissues, and 33.33% in adjacent tissues. Sp1 staining in cancer tissue was positively correlated to TNM stage （ r=0.349, P〈0.05） , tumor invasion （ r=0.407, P〈0.01） , and lymph node metastasis （r=0.314, P〈0.05）. Univariate analysis indicated that the overall survival rate was significantly lower in Sp1-positive patients than in Sp1- negative patients （41.86% vs. 82.35% , P〈0.05）. Cox multivariate analysis showed that Sp1 expression, TNM stage, invasion and lymph node metastasis were independent prognostic factors of breast cancer. CONCLUSIONS： Sp1 maybe participate in the invasion and metastasis of breast cancer, and is one of the valuable markers indicating poor prognosis of breast cancer. Sp1 detection, with consideration of tumor invasion and clinical stage, may increase the accuracy of predicting prognosis of patients with breast cancer.