东乡野生稻育性恢复性的鉴定与遗传分析

Identification and Genetic Analysis of Fertility Restoration in Dongxiang Wild Rice

以东乡野生稻水桃树、东塘上2个居群与不同细胞质来源的5个水稻不育系（B06S、珍汕97A、协青早A、中97A和粤泰A）配组，根据F1结实率的高低对东乡野生稻育性恢复性进行鉴定。建立了10个组合的P1、F1、P2、F2群体，利用混合模型理论的Akaike信息准则（AIC），在F2代中鉴定影响数量性状的主基因存在与否，主基因存在时通过分离分析估计主基因和微效基因的遗传效应以及所占总变异的分量。F1代的结实率变化范围为45．98％～76．57％，表明东乡野生稻具有一定的育性恢复性。F2代中该性状符合1对主基因＋多基因的遗传模式，主基因遗传率为56．63％～88．29％，多基因遗传率为2．74％～30．97％，总基因型遗传率为63．17％～94．01％。中9A／东塘上居群杂交组合的F2代中，主基因是加性遗传，无显性效应，其他9个组合主基因是完全显性遗传。

Two populations of Dongxiang wild rice from Shuitaoshu and Dongtangshang were crossed with five male sterile rice lines with different cytoplasmic backgrounds, and the seed setting rate of the F1 was used to judge the existence or not of the fertility restoration in the Dongxiang wild rice. With P1 , F1 , P2 , F2 populations, the Akaike＇s information criterion （AIC） was used to identify the existence of major genes affecting quantitative traits, and when the major genes existed, the genetic effects of the major genes and polygenes and their genetic variance were estimated through segregation analysis. The seed setting rates of the F1 generations varied from 45.98% to 76.57%, suggesting that the Dongxiang wild rice had the ability of fertility restoration. One major gene and polygene mixed model was the most fitted genetic model for this trait in all the F2 populations. The heritability values of the major genes varied from 56.63% to 88.29 % and those of the polygenes varied from 2.74% to 30.97%, and the total heritability values were from 63.17% to 94.01%. The major gene inheritance of the combination Zhong 9A/Dongtangshang was controlled by the additive effect without dominant effect, and the other nine com binations were complete dominant inheritance.