水稻印尼水田谷型细胞质雄性不育恢复系R68的恢复基因初步定位

Mapping of Rice Fertility-Restoring Gene for Yinshui Cytoplasmic Male Sterility in a Restorer Line R68

用印尼水田谷型不育系中9A和恢复系R68配组,选取F2的高可育株和极端不育株构建2个基因池,用82个完全不育单株作为定位群体,利用分布于12条染色体的413对SSR引物对双亲和两池进行多态性分析。位于第1染色体的RM283和位于第10染色体的RM5756、RM258、RM6100、RM171在亲本、两池间存在多态性,用F2单株验证证明它们与恢复基因连锁。经典遗传分析和分子标记定位研究表明,印尼水田谷型细胞质雄性不育恢复系R68具有2对恢复基因,分别位于第1和第10染色体上。位于第1染色体的恢复基因与分子标记RM283的距离是6.7 cM,位于第10染色体的恢复基因与标记RM5756、RM258、RM6100和RM171间的距离分别是10.4、8.0、2.4和4.2 cM。

An F2 population derived from the cross of Zhong 9A/R68 was used to map the fertility-restoring （R f） gene for Yinshui cytoplasmic male sterility （CMS）. A fertile bulk was constructed by pooling equal amount of 10 highly fertile lines and a sterile bulk was obtained by pooling equal amount of 10 highly sterile lines. Four hundred and thirteen pairs of SSR primers, which distributed on 12 chromosomes, were screened for polymorphism between the parents and between the two bulks. RM283 on chromosome 1 and RM5756, RM258, RM6100, RM171 on chromosome 10 were found to be polymorphic between the parents and between the two gene bulks. Ten highly fertile and ten highly sterile lines were selected from F2 population of Zhong 9A/R68 to screen the gene bulks. The results showed that the five SSR markers were linked to fertility-restoring genes. A total of eighty two-excessive sterile lines were selected from Zhong 9A/R68 F2 population to estimate the genetic distance between the five SSR markers and fertility restoring genes respectively. A Rf gene was located on chromosome 1 with a distance of 6.7 cM to RM283, and the otherRf gene was mapped on the long arm of chromosome 10 flanked by RM5756, RM258, RM6100 and RM171 at distances of 10.4, 8.0, 2.4 and 4.2 cM, respectively.