胀果甘草悬浮培养细胞合成甘草总黄酮(英文)

Production of Flavonoids in Cell Suspension Culture of Glycyrrhiza inflata (Leguminosae)

比较了胀果甘草(Glycyrrhiza inflata)悬浮细胞在逐级放大摇瓶中的生长、黄酮产量以及营养消耗过程,以便了解其放大规律。结果表明,在250和500mL摇瓶中,细胞的最大生物量、黄酮产量以及最大比生长速率没有显著性差异,但是在1L的摇瓶中,这三种参数都显著地降低,分别比250mL摇瓶中降低了27%,30%和27%。在逐级放大的摇瓶中,氮、磷、铵浓度都随着培养时间延长而逐渐降低,尽管在1L的摇瓶中磷消耗得最慢,但三种摇瓶中磷在细胞生长对数期基本都被消耗尽了。此外,硝态氮在第18天时基本被消耗完,而铵态氮在细胞收获时仍能维持在100mg/L。因此在反应器中培养时,主要的培养条件还需进一步优化。

The cell biomass, flavonoids production and nutrients consumption were monitored to characterize the culture of progressive scale-up of Glycyrrhiza inflata. The maximum biomass, flavonoids production and the maximum specific growth rate in the culture of 250 and 500 mL flasks remain similar, but were significantly higher than that of 1 000 mL flask. The three parameters in the culture of 1 000 mL flask were 27 %, 30%, and 27 % lower than that of 250 mL flask, respectively. The concentrates of phosphate, nitrate and ammonium in progressive scale-up flasks decreased similarly. Phosphate and nitrate were almost exhausted on day 10 and 18 in all flasks, respectively; while ammonium maintained about 100 mg/L till cells were harvested. The basic culture conditions needed to be further optimized for higher flavonoids production on a bioreactor scale.