福州地区无菌性脑膜炎病原——埃柯病毒30型分子流行病学分析

Molecular Epidemiology of ECHO Virus Type 30 Isolated From an Aseptic Meningitis Outbreak in Fujian Province

目的研究福州地区2004年无菌性脑膜炎病原——埃柯病毒30型(ECHO30)VP1区基因特征及其分子流行病学特点。方法选取福州地区2004年2株ECHO30(FJ-C2-04、FJ-C7-04),进行核糖核酸(RNA)提取和VP1区逆转录-聚合酶链反应(RT-PCR),测定和分析PCR产物的核苷酸序列。结果所测2株ECHO30VP1区基因全长都是876bp,翻译的氨基酸全长292aa。2株病毒VP1区核苷酸同源性为99.89%(仅相差1个bp),氨基酸序列完全相同。FJ-C2-04株VP1区基因全长和基因库(GenBank)上检索的相关ECHO30毒株比较,同源性为75.66%～97.49%。构建的ECHO30遗传进化树将已知的ECHO30划分为7个组,福州地区分离的ECHO30在第7组。结论ECHO30随着年代沿革不断衍化,福州地区分离株与早期分离株差异最大,与2004年浙江省分离株亲缘关系最近。2002～2004年在浙江、福州和2003年山东、江苏省存在引起无菌性脑膜炎的ECHO30的局部流行。

Objective To study the molecular characterization of ECHO virus type 30 （ECHO30） during an outbreak of aseptic meningitis in Fuzhou City,Fujian Province in 2004. Methods To determine the complete nucleotide sequence of VP1 region of the etiologic virus （2 ECHO30） strains-FJ-C2-04 and FJ-C7-04）, and analyze the homogeneity, then construct the phylogenetic tree based on complete VP1 region of ECHO30. Results The complete nucleotide sequence of VP1 region of these ECHO30 isolates was 876nt coding 292 amino acids. The homogeneity was 99.89% between FJ-C2-04 and FJ-C7-04,and 75.66-97.49% between FJ-C2-04 and the ECH- O30 reference strains. The phylogenetic tree of ECHO30 wed 7 groups,and FJ-C2-04 and FJ-C7-04 belong to Group 7. Conclusion The deduction is there were two independent circulation of ECHO30 in Fujian/Zhejiang Province and Shandong/Jiangsu Province which caused respectively an endemic of aseptic meningitis in local areas in the period of 2002 to 2004.