辽宁省1990～2005年非脊髓灰质炎肠道病毒逆转录-聚合酶链反应鉴定及其病例的流行病学分析

Identification for Non-polio Enteroviruses by Reverse-transcription Polymerase Chain Reaction and Epidemiological Analysis on Cases From 1990 to 2005

目的了解辽宁省急性弛缓性麻痹(AFP)病例中非脊髓灰质炎肠道病毒(NPEV)感染的情况。方法用逆转录-聚合酶链反应(RT-PCR)对NPEV进行鉴定,并对NPEV感染病例进行流行病学分析。结果辽宁省1990～2005年AFP病例中共有98例NPEV感染病例,NPEV分离率为7.8%。其中67.3%的病例为4岁以下儿童;59.2%的病例出现在6～8月;主要临床表现有发热(56.2%)、肌肉疼痛(29.6%)、腹泻(12.2%);58.2%的NPEV感染病例被诊断为格林-巴利综合征(GBS)。结论用RT-PCR方法鉴定NPEV快速、简单、经济、特异性强;NPEV可能是GBS的重要病因之一。

Objective To know the non-polio enteroviruses （NPEV） infection of the acute flaccid paralysis （AFP） cases. Methods NPEV were identified by reverse-transcription polymerase chain reaction （RT-PCR） and epidemiological analysis were made in these NPEV cases. Results From 1990 to 2005,altogether 98 NPEV strains were isolated from AFP cases and the isolation rate was 7.8%. 67.3% of cases were children younger than 4 years old;59.2% of cases appeared in June to August; The clinic symptoms had fever （56.2%）, myalgia （29.6%） and diarrhea （12.2%） ;58.2% of cases were diagnosed as Guillain-Barre Syndrome （GBS）. Conclusion The Rt-PCR method was rapid, simple, economical, and high specific for identification of NPEV; NPEV infection was probably responsible for GBS.